Association Between PGLYRP2 Gene Polymorphism And Sporadic Parkinson’s Disease In The Northern Chinese Han Population

Objective:Gut inflammation is increasingly corroborated to take part in the pathogenesis of Parkinson’s disease(PD).The PGLYRP2 gene has been proven to have the susceptibility to inflame bowel disorder.The present study aimed to explore the genetic relationship between single nucleotide polymorphism(SNP)of the PGLYRP2 gene and the risk of sporadic PD in the Han population in northern China.Methods:This is a case-control experiment.The case group consisted of 400 northern Chinese Han PD patients,and the control group consisted of 400 age-and gender-matched healthy individuals with no family history of PD or neurodegenerative diseases.PD patients were divided into two groups: the early-onset PD(EOPD)group of patients 50 years old or younger and the late-onset PD(LOPD)group of patients older than 50 years old.DNA was extracted from the patients’ venous blood,and the genotypes of the rs3813135 T/C,rs733731 C/T and rs892145 A/T polymorphisms of PGLYRP2 were identified by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism(PCR–RFLP)method.The data were analyzed using the SPSS Statistics software(26.0 version).The differences in genotype and allele frequencies between the groups was compared in a Chisquare test.The relationships between the polymorphisms and PD susceptibility were evaluated through the 95% confidence interval(95% CI)and odds ratio(OR),and all comparisons were separately and collectively corrected for gender and age by logistic regression.Differences with P <0.05 were considered as statistically significant.Results:The results showed that the demographic data of the two groups were in line with Hardy-Weinberg balance,and there was no statistical difference in gender and age between the two groups and subgroups(P>0.05).In the statistical analysis of rs892145,the frequency of AT genotype in the case group was higher than that in the control group,and there was a significant difference between the two groups(OR=1.459,95%CI=1.459-1.039,P=0.029),as well as early-onset PD and control groups(P=0.024).There was no statistical difference in the comparison of other alleles or genotypes and the comparison between subgroups.In rs3813135,there was only one meaningful result in the subgroup analysis:the frequency of the C allele in the male case group was significantly higher than that in the male control group(P=0.045).The comparison between the frequencies of other alleles and genotype was not statistically different.Conversely,no significant difference in rs733731 was found between the PD and healthy participants.Linkage Disequilibrium(LD)was detected and showed that the three SNPs of PGLYRP2 were in strong LD,which indicated a potentially strong linkage effect of three SNPs on mutations eliciting functional changes.Five common haplotypes were checked,of which the haplotypes TTA and TCA were related to PD susceptibility.Conclusion:In summary,our results indicated that the PGLYRP2 gene was related with PD in the Chinese Han population,in which the rs892145 AT heterozygote might increase the risk of PD and possibly the risk of early-onset PD.The C allele of rs3813135 may increase the risk of PD in male;while rs733731 is not associated with PD.