Study On Photothermal Therapy Of Breast Cancer Based On PB@PDA Core-shell Nanocomposites

Objective :To explore the photothermal effect of PB@PDA nanocomposites,and to preliminarily explore the biosafety compatibility and photothermal toxicity of PB@PDA in breast cancer cells.Using Prussian blue(PB)and polydopamine(PDA)as photothermal agent(PTA)properties,provide experimental basis for breast cancer photothermal therapy(PTT).Methods: In this project,We synthesized PB@PDA nanocomposites,and in order to fully demonstrate the advantages of the composites,we compared the photothermal effects of PB@PDA composites with PB alone and the therapeutic effects of breast cancer.The morphology of the material was characterized by electron microscopy(SEM,TEM),the particle size of the material was measured by dynamic light scattering test(DLS),and the structure of the material was detected by Fourier transform infrared spectroscopy(FT-IR)and X-ray diffraction(XRD).Subsequently,Photothermal effects of the materials were evaluated under 808 nm NIR irradiation.The effects of concentration,irradiation time and laser intensity on the photothermal properties of the material were further explored.And through the repeated heating and cooling cycles of the material,the stability of the photothermal properties of the material was evaluated.After evaluating the biocompatibility of the material,further in vitro experiments were conducted to verify the photothermal effect of the material on 4T1 tumor cells.The photothermal therapy effect of the materials at the cellular level was evaluated and explored and live and dead cell double staining method(Calcein-AM/PI).Results:(1)Two nanomaterials,PB and PB@PDA,which meet the needs of the biomedical field,were successfully synthesized.(2)PB and PB@PDA nanomaterials have a certain photothermal effect,which is dependent on concentration,laser intensity,and irradiation time;(3)PB,PB@PDA nanomaterials have certain photothermal stability;(4)PB,PB@PD nanomaterials have excellent biocompatibility in the working concentration range;(5)PB,PB@PDA nanomaterials have certain The photothermal toxicity of cells was concentration-dependent.Conclusion:(1)Our results show that both PB and PB@PDA nanomaterials have certain photothermal properties,and can eliminate breast cancer 4T1 cells under light conditions;(2)At the same time,it is shown that the PB@PDA material is more effective than PB in PTT.Through the research of this topic,we demonstrated that the two nanomaterials,PB and PB@PDA,can be irradiated with a specific intensity of NIR laser at a concentration that does not damage normal cells,resulting in a photothermal effect,increasing local temperature to kill tumor cells.The results indicated that the PB@PDA nanocomposite has the potential to become a new type of photothermal agent,which has application value in photothermal therapy.