| Objective:Age-related macular degeneration is a multifactorial diseasse and the main disorder of blindness with unknown etiologies in developed countries.Oxidative stress is thought to be associated with the AMD progression,however,relatively little is known about the potential diagnostic value of oxidative stress related genes in AMD.Moreover,its role in regulating AMD immune microenvironment has not been studied yet.Method:The OS genes with abnormal expression between AMD and normal human samples were screened.Characteristic genes were identified and selected by univariate logistic regression,LASSO,and SVM.The diagnostic model was constructed via the multivariate logistic regression.The imparts of non-late and late AMD on the immune microenvironment characteristic were explored including infiltrating immune cells,immune response gene-sets,HLAs(human leukocyte antigen),and complement genes,.Subtypes mediated by the identification of os key genes were systematically evaluated in AMD samples.Subtype-related genes and the immune-related genes were identified by differential expression.Functional and Gene Ontology analyses were also performed.Result:13 significant oxidative stress genes were obtained in the differential analysis of healthy between AMD samples,indicating that they were enriched in immune-related pathways.Six hub genes were obtained from the PPI network.Five key genes were obtained by identifying for a diagnostic model with an ROC area under the curve of 0.875.The effects of non-late and late AMD on the characteristics of the immune microenvironment,including infiltrating immune cells,immune response gene sets,HLAs(human leukocyte antigens),and complement genes.Two subtypes mediated by the identification of 5 key genes were systematically evaluated in AMD samples.They differ in immune cell abundance,immune response,and complement genes.351 subtype-related genes and 48 immune-related genes were identified under functional and Gene Ontology(GO)analysis to reveal potential pathways enriched in these genes.Also in the WGCNA analysis,the hub genes of the isoform-1-related modules were identified.Conclusion : This study identified five key biomarkers of oxidative stress contributing to the diagnosis of AMD,and constructed a diagnostic model with good predictive effect.The acquisition of hub genes associated with key genes of oxidative stress-mediated subtypes simultaneously revealed potential oxidative stress-related regulatory mechanisms in the immune microenvironment of AMD. |
