Mechanism And Clinical Significance Of The MiR17-92 Cluster In Gout

Objective:To explore the expression of each members of the miR17-92 cluster in peripheral blood mononuclear cells(PBMCs)of patients in gouty arthritis,predict their possible targets and pathways of action,and to evaluate their possible mechanism and clinical significance in gout.Methods:A total 67 gouty arthritis(GA)patients were selected,including 22 patients with acute gout(AG)and 45 patients with intermittent gout(IG),and 35 normal health control(HC)were selected in our hospital.RT-qPCR measured the expression of miR 17-92 cluster.The miR17-92 cluster related target genes were searched and screened out by bioinformatics and other methods.RT-qPCR measured the relative expression of IFN-y,IL-10 and some members of JAK-STAT pathway through RT-PCR,and relevant laboratory indicators were collected to analyze the correlation between each other.Results:The relative expression of miR 17,miR 18a,miR19a,miR20a,and miR19b were significantly changed in AG,IG and HC(H=8.753,P<0.05;H=6.338,P<0.05;H=6.523,P<0.05;H=9.061,P<0.05;H=9.729,P<0.01).The expression of JAK3 and STAT2 was statistically different in the AG,IG and HC groups(H=10.349,P<0.01;H=14.801,P<0.01).Expression of IFN-γwas statistically different among the AG,IG and HC groups(H=8.734,P<0.05).In AG patients,miR18a expression was inversely correlated with IBIL,Crea,MO,and HGB.miR 19a expression was negatively associated and TC,UA,and HGB.miR20a expression was negatively associated with Crea.miR19b expression was negatively associated with UA and HGB.In IG patients,miR 17 expression was negatively associated with IBIL,WBC,LY,and MO.miR18a expression was positively associated with ALP,miR19a expression was negatively associated with TC and UA,and miR20a expression was negatively associated with ADA and UA.Conclusion:The miR17-92 cluster may regulate the development and participate in the clinical pathology of gout by targeting the JAK-STAT pathway.