The Relationship Between Revascularization Surgery And Plasma Cytokines And LncRNA MEG3 Expression In Patients With Ischemic Moyamoya Disease

Objective: Moyamoya Disease(MMD)is characterized by stenosis of internal carotid artery with formation of "smoke-like" vascular network at the skull base.It is very important to clarify the cause of pathological mechanism.Many cytokines in vivo regulate the formation of blood vessels,among which VEGF,bFGF,PDGF and MMPs all play an important role.However,lncRNA MEG3 can play a role by regulating VEGF-related pathways.This study analyzed the possible pathogenesis by exploring the expression of MMD-related factors and MEG3 of blood deficiency type.Methods: The patients with ischemic MMD who received revascularization and the healthy subjects were divided into preoperative MMD group,postoperative MMD group and healthy control group according to whether they received surgical treatment.The peripheral blood of the subjects was extracted and the plasma was separated.The concentrations of VEGF,bFGF,PDGF-BB and MMP-9 in the subjects’ plasma were detected by Elisa.Then the expression of lncRNA MEG3 in the plasma of three groups of subjects was detected by q RT-PCR,and the expression relationship between MEG3 and various factors was compared.Results: The expression of VEGF,bFGF,PDGF-BB,and MMP-9 in the plasma of patients with ischemic MMD was significantly higher than that of healthy individuals.The expression of these four factors remained high after surgical treatment,but there was no significant difference compared to before surgery;lncRNA MEG3 also exhibits high expression in plasma,indicating that MEG3 may participate in the occurrence and development of MMD by regulating changes in VEGF and PDGF-BB,thereby mediating the formation of blood vessels.Conclusion: The expression of VEGF,bFGF,PDGF-BB and MMP-9in the plasma of patients with MMD of blood deficiency type was significantly higher than that of healthy people.The expression of the four factors was still higher after surgery,but there was no significant difference between the two groups;lncRNA MEG3 is also highly expressed in plasma,which indicates that MEG3 may participate in the occurrence and development of MMD by regulating the changes of VEGF and PDGF-BB,thus mediating the formation of blood vessels.