The Expression Of UBE2B In Esophageal Cancer And Its Relationship With Epithelial-mesenchymal Transition And Metastasis

Objective:To explore the expression of UBE2B in esophageal carcinoma and its relationship with epithelial-mesenchymal transition and metastasis.Methods:The relationship between the level of UBE2B mRNA and the clinicopathological characteristics of esophageal cancer and its influence on the prognosis was examined using the TCGA database and bioinformatic analysis.Immunohistochemistry was used to detect the protein expression level of UBE2B in esophageal cancer and adjacent tissues.The si-UBE2B and control si-Control plasmids were transfected into esophageal cancer cells Eca-109 by liposome transfection method.Cell Transwell experiments were performed to detect cell migration ability.Western blotting was conducted to assess the effect of low expression of UBE2B on EMT-related marker proteins.Results:The results of TCGA bioinformatics analysis showed that the expression level of UBE2B in esophageal cancer was significantly higher than that in normal esophageal cancer tissues,and the survival of patients with high UBE2B expression was significantly worse than that of patients with low UBE2B expression.The results of esophageal cancer tissue microarray immunohistochemistry showed that UBE2B protein was significantly expressed in esophageal cancer.The Transwell test indicated that ECA-109 cells migration ability was significantly inhibited after si-UBE2B was transfected into ECA-109 cells.Knockdown of UBE2B increases the expression of EMT-related protein E-cadherin protein,while inhibiting the expression of N-cadherin,Slug and Snail proteins.Conclusions:UBE2B is abnormally highly expressed in esophageal cancer and is significantly related to poor prognosis.Knockdown of UBE2B inhibits cell migration and invasion by inhibiting the epithelial-mesenchymal transition of esophageal cancer cells.