Study On Function And Mechanism Of LINC01936 In Lung Squamous Cell Carcinoma

Objective Study the biological function and potential mechanism of Long Non-coding RNA(Lnc RNA)LINC01936 in Lung Squamous Cell Carcinoma(LUSC).Methods Transcriptome data of LUSC from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases with DEseq2,Edge R and Limma in R language was used to analyze the differentially expressed Lnc RNA in LUSC and adjacent tissues.The relationship between LINC01936 and clinicopathological features and prognosis of LUSC patients was analyzed by online databases.The localization of LINC01936 in LUSC cells was predicted by the lnc Locator.The proliferation and apoptosis of LUSC cells was investigated by CCK-8 and fluorescence stain,respectively.The migration and invasion were detected by Transwell assay.The function and pathway enrichment analysis were performed by Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Set Variation Analysis analysis(GSVA).The relationship between LINC01936 and tumor immune microenvironment was analyzed by ESTIMATE algorithm.Results In TCGA database,the 2139 differentially expressed Lnc RNA(1603upregulated,536 downregulated),2138 differentially expressed Lnc RNA(1702upregulated,436 downregulated)and 1159 differentially expressed Lnc RNA(529upregulated,630 downregulated)were obtained by the Deseq2,edge R and Limma,respectively.In GEO database,we obtained 341 differentially expressed Lnc RNA(206upregulated,135 downregulated).A total of 52 common differentially expressed Lnc RNA were obtained by intersection of the above differentially expressed Lnc RNA.We found that LINC01936 existed in the cytoplasm of LUSC cells,and significantly downregulated in LUSC tissues.LINC01936 was significantly associated with early clinical stage,N stage and poor prognosis of LUSC patients.The overexpression of LINC01936 in vitro could significantly reduce the proliferation,migration and invasion ability in LUSC cells,and increase cell apoptosis.The co-expressed genes with LINC01936 were obtained and enriched to cell cycle pathway.The genes in LINC01936 high expression group activated complement,JAK-STAT3 signalling,NF-κB signalling,epithelial-mesenchymal transition and angiogenesis.LINC01936 was positively correlated with stromal score,immune score,ESTIMATE score and infiltrating immune cells in LUSC.Conclusion LINC01936 is down-regulated in LUSC and significantly correlated with clinical stage,N stage and prognosis of LUSC patients,the overexpression of LINC01936 can regulate the proliferation,apoptosis,invasion and migration of LUSC.LINC01936 maybe regulate tumor-related pathways to affect the occurrence and development of LUSC,and provide a new target for clinical treatment of LUSC.Figure 16;Table 8;Reference 133...