The Expression And Clinical Significance Of Long Nondcoding RNA In Stevens-Johnson Syndrome And Toxic Epidermal Necrolysis

Background:Stevens-Johnson Syndrome(SJS)and Toxic Epidermal Necrolysis(TEN)are a group of rare but life-threatening severe cutaneous adverse reactions.However,the specific pathogenic mechanisms of SJS/TEN remain unclear,which makes it challenging,arduous and concerning to seek out biomarkers for early diagnosis and treatments.Studies have confirmed that long non-coding RNAs(lncRNA)involved in cell development,proliferation and apoptosis through regulating gene expression.In this study,we aim to investigate the potential biological functions of lncRNAs in SJS/TEN.Objective:To construct the differential expression profile of lncRNAs in PBMC of SJS/TEN patients and healthy controls,explore its clinical significance on the diagnosis and treatment of SJS/TEN,and to provide a theoretical basis for further exploring the mechanism of SJS/TEN.Methods:Using high-throughput seuquencing to identify the differential expression profiles of lncRNAs between 3 pairs of SJS/TEN patients and healthy controls.GO and KEGG enrichment analysis presented gene functional features.Four candidate lncRNAs were verified by qRT-PCR.An examination of the correlation between candidate lncRNAs in PBMCs and the severity of SJS/TEN was conducted using ROC curve to assess the diagnostic value.Moreover,ceRNA regulatory networks of candidate IncRNA were constructed to provide a hypothesis for the pathogenesis of SJS/TEN.Results:1.A total of 1855 lncRNAs(462 up-regulated and 393 down-regulated)were identified,based on the screening criteria.2.GO and KEGG functional enrichment analysis showed that the differential expressed genes were mainly associated with T-lymphocyte differentiation,NK cell mediated cytotoxicity.3.Compared with control group,the expression level of HCG18 was significantly decreased(p<0.05)and that of lncRNA LOC102724545 was significantly upregulated(p<0.05)in PBMCs of SJS/TEN group.4.The ROC curve suggested that HCG18 and LOC102724545 could be realiable diagnostic makers for SJS/TEN,with AUCs of 0.9504(95%CI:0.87-1.00,p<0.001)and 0.7934(95%CI:0.59-0.99,p<0.05),respectively.5.Correlation analysis suggested that the relative expression of HCG18 was negatively correlated with SCORTEN(r=-0.7551,p<0.01)and RDW/Hb(r=0.8818,p<0.001).Conclusion:1.In this study,there are significantly differentially expressed lncRNAs in PBMCs of SJS/TEN patients by second-generation high-throughput sequencing technology.2.The lncRNA HCG18 and lncRNA LOC102724545 have potential diagnostic value for SJS/TEN,and HCG18 may serve as a potential biomarker reflecting the severity of SJS/TEN.