The Expression And Biological Function Of RBBP9 In Colorectal Cancer

Objective:In this study,we investigated the expression of RBBP9 in colorectal cancer,and further verified the relative expression level of RBBP9 in cancer tissues and adjacent tissues.Subsequently,we conducted cell function experiments to explore the effect of the expression level of RBBP9 on the biological function of colorectal cancer cells.Methods:1.15 pairs of fresh colorectal cancer and its adjacent tissues were detected by two-dimensional liquid chromatography-mass spectrometry,and a series of up-regulated and down-regulated proteins were obtained.Western Blot was used to further verify that the selected protein RBBP9 was highly expressed in colorectal cancer tissues compared with adjacent tissues.2.The biological company assisted in the construction of the plasmid.After obtaining the plasmid,I transformed the plasmid into EScherichia coli,purified and cultured it,and shook the bacteria to extract the plasmid for the next step of cell function experiment transfection preparation.3.The plasmid was transfected into colorectal cancer cells with non-liposome transfection reagent,and the transfection efficiency was observed by fluorescence microscope.The transfection efficiency was verified by RT-QPCR and Western blot.The effect of RBBP9 on colorectal cancer cell proliferation was detected by CCK8 cell proliferation assay.Transwell detected the effects of RBBP9 on the migration and invasion of colorectal cancer cells.Annexin V-PE/7AAD double staining flow cytometry was used to analyze the effect of RBBP9 on apoptosis of colorectal cancer cells.Results:1.The differentially expressed proteins in colorectal cancer tissues were analyzed by two-dimensional liquid chromatography-mass spectrometry,and RBBP9 was identified as one of the up-regulated proteins.After data query,I determined that RBBP9 was less studied and of certain significance for further research,and it was determined as my research topic.Moreover,western blot was used to verify the up-regulated expression of RBBP9 in colorectal cancer tissues compared with para-cancer tissues.2.RBBP9 overexpression plasmid(ov RBBP9),negative control plasmid(ov Ctrl)and silencing plasmid(shr BBP9-E,shr BBP9-F,shr BBP9-F,shr BBP9-F,shr BBP9-E,SHRBBP9-F,SHRBBP9-F,shr BBP9-E,SHRBBP9-F,SHRBBP9-F,SHRBBP9-E,SHRBBP9-F,SHRBBP9-F,SHRBBP9-E,SHRBBP9-F,SHRBBP9-E,SHRBBP9-F,Shrbbp9-g)and its negative control plasmid(sh Ctrl);Rt-qpcr and Western Blot were used to verify the successful construction of SW620 cell lines with overexpression and knockdown expression of RBBP9.We explored the effects of RBBP9 on cell biological functions through cell function experiments:overexpression of RBBP9 can promote cell proliferation,migration and invasion and inhibit apoptosis,while silencing RBBP9 can inhibit cell proliferation,migration and invasion and promote apoptosis.Conclusions:1.RBBP9 is up-regulated in colorectal cancer tissues.2.Up-regulated expression of RBBP9 can promote the proliferation,migration and invasion functions of COLORECTAL cancer cells and inhibit apoptosis,while silencing RBBP9 can inhibit the proliferation,migration and invasion functions of colorectal cancer cells and promote apoptosis.RBBP9 may serve as a potential biomarker for the diagnosis and treatment of colorectal cancer.