LncRNA HOTTIP Promotes The Development Of Ovarian Cancer By HIF-1α In Hypoxic Environment

Objective:This study aims to study the expression levels,relationship and biological functions of LncRNA HOTTIP and HIF-1α in ovarian cancer cells under hypoxia environment,providing a certain research basis for exploring the pathogenesis of ovarian cancer,and providing new ideas and theoretical basis for finding new targets for the diagnosis and treatment of ovarian cancer.Methods:1.Expression of LncRNA HOTTIP in ovarian cancer cells and normal ovarian epithelial cells under hypoxia environment was detected by RT-qPCR.After LncRNA HOTTIP was knocked down and overexpressed in ovarian cancer cells,LncRNA HOTTIP and HIF-1αm RNA levels were detected by RT-qPCR.Western Blotting was used to detect HIF-1α protein levels in ovarian cancer cells.The correlation between LncRNA HOTTIP and HIF-1α was analyzed.2.The migration and invasion of ovarian cancer cells after knockdown and overexpression of LncRNA HOTTIP in hypoxia environment were detected by Transwell assay,the proliferation of ovarian cancer cells was detected by CCK-8,and the cell cycle and apoptosis of ovarian cancer cells were detected by flow cytometry.Results:1.The expression of LncRNA HOTTIP in ovarian cancer cells was higher than that in normal ovarian epithelial cells under hypoxia environment(P < 0.001).The expression of HIF-1α m RNA and HIF-1αprotein in ovarian cancer cells decreased after LncRNA HOTTIP knocked down(P<0.01).After overexpression of LncRNA HOTTIP,HIF-1α m RNA and HIF-1α protein expression in ovarian cancer cells increased(P <0.01).And LncRNA HOTTIP was positively correlated with HIF-1αexpression(|r| = 0.9873).2.In the hypoxia environment,LncRNA HOTTIP promoted the proliferation,invasion and metastasis of ovarian cancer cells and inhibited their apoptosis.Ovarian cancer cells with high expression of LncRNA HOTTIP had enhanced invasion and migration ability(P < 0.05),increased cell viability(P < 0.0001)and decreased apoptosis rate(P <0.05).Conclusion:LncRNA HOTTIP targets HIF-1α to promote the development of ovarian cancer in hypoxia environment.