TRIB3 Promotes The Development Of Nasopharyngeal Cancer Cells By Activating The β-catenin Signaling Pathway

Objective: This study aims to explore the mechanism of action of TRIB3 in nasopharyngeal cancer cells,how to change the malignant biological behavior of nasopharyngeal cancer cells,and how to influence the occurrence and development of nasopharyngeal cancer cells by activating the β-catenin signaling pathway,in order to obtain more effective therapeutic effects.Methods: The q RT-PCR assay was used to detect the relative expression level of TRIB3 in normal nasopharyngeal epithelial cells(N69)and nasopharyngeal cancer cells(HNE-1,CNE-2Z,5-8F).Human nasopharyngeal cancer cells HNE-1 and CNE-2Z were cultured in vitro,and each cell was divided into two groups,namely the negative control group(NC group)and the knockdown TRIB3 group(sh-TRIB3 group),and the expression level of TRIB3 in each group of nasopharyngeal cancer cells after transfection was detected by q RT-PCR experiment and Western blot experiment.After knocking out TRIB3,EDU experiment,CCK-8 experiment and cell cloning experiment detected the proliferation ability of nasopharyngeal cancer cells,Transwell experiment and scratch experiment detected the invasion and migration ability of nasopharyngeal cancer cells,flow cytometry detected apoptosis of nasopharyngeal cancer cells,Western blot experiment detected EMT-related proteins Snail,E-cadherin,Relative expression levels of N-cadherin and Vimentin and β-catenin signaling pathway-related proteins GSK-3β,p-GSK-3β,β-catenin,p-β-catenin.Results: The results of PCR showed that compared with normal nasopharyngeal epithelial cells(N69),the expression of TRIB3 in nasopharyngeal cancer cells(HNE-1,CNE-2Z)was significantly increased(P<0.01),and two nasopharyngeal cancer cells were subsequently selected for experiments.Through q RT-PCR experiments and Western blot experiments,it was found that in HNE-1 and CNE-2Z cells,the relative expression of TRIB3 in sh-TRIB3 group was downregulated(P<0.05)compared with the NC group.Through EDU experiments,CCK-8 experiments and cell cloning experiments,it was found that in HNE-1 and CNE-2Z cells,the proliferation ability of nasopharyngeal cancer cells in the sh-TRIB3 group was significantly inhibited compared with the NC group(P<0.05).The results of Transwell experiment and scratch experiment showed that in HNE-1 and CNE-2Z cells,the migration and invasion ability of nasopharyngeal cancer cells in the sh-TRIB3 group was significantly inhibited compared with the NC group(P<0.05).The results of flow cytometry detection showed that in HNE-1 and CNE-2Z cells,the apoptosis nasopharyngeal cancer cells in the sh-TRIB3 group increased significantly compared with the NC group.Through the results of Western blot experiments,it was found that in HNE-1 and CNE-2Z cells,compared with the NC group,the expression of EMT-related proteins E-cadherin in the sh-TRIB3 group was upregulated,the expression of Snail,N-cadherin and Vimentin was downregulated(P<0.05),the expression of p-GSK-3β and β-catenin proteins associated with the β-catenin signaling pathway was down-regulated,and the expression of GSK-3β and p-β-catenin was upregulated(P<0.05).Conclusion: In nasopharyngeal cancer cells,TRIB3 is in a high expression state,and knocking down TRIB3 can inhibit the proliferation,migration and invasion ability of nasopharyngeal cancer cells and promote apoptosis of nasopharyngeal cancer cells.Therefore,the mechanism of action of TRIB3 in nasopharyngeal carcinoma may be an effective anti-tumor target,which can effectively inhibit the growth and spread of nasopharyngeal cancer cells by regulating the β-catenin signaling pathway,thereby providing new therapeutic ideas for clinical treatment.