| Objective: To investigate the differences in the abundance of Akkermansia muciniphila(AKK)in patients with alcohol use disorder in different nationnalities and the relationship with ALD.Methods: Patients of Han and Uyghur ethnicity who attended the First Affiliated Hospital of Xinjiang Medical University and Xinjiang Mental Health Center were recruited to collect clinical information,including basic demographic information,a self-administered alcohol use questionnaire,and an assessment of alcohol dependence severity using the Alcohol Use Disorders Screening Inventory(AUDIT).Biological samples,including EDTA plasma and blood cells and stool samples,were collected for subsequent processing in the laboratory and frozen in-80 C refrigerators,respectively.EDTA plasma samples for liver and kidney function tests and assessment of the severity of liver and kidney function.Detection of flora species and relative abundance by 16 Sr RNA sequencing in fecal samples.Blood cell samples were genotyped for alcohol metabolizing enzymes by extracting genomic DNA.Results:Chronic alcohol dependence can lead to a significant decrease in the alpha diversity of the flora.At the phylum level,the phylum Actinobacteria was significantly lower in the Han and Uyghur alcohol-dependent populations;genus and species level,Akkermansia and A.muciniphila were significantly lower in the alcohol-dependent population;Long-chain fatty acids were significantly reduced in the Han and Uyghur alcohol-dependent populations.The species-level beta diversity of the Han population is associated with AST and GGT,while the Uyghur population is associated with total bilirubin and indirect bilirubin.Conclusion: 1.The gut microbiota composition differed between Han and Uyghur alcohol-dependent and control populations.2.AKK was significantly reduced in Han alcohol-dependent populations.3.Liver injury was associated with low-level long-chain fatty acids. |
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