Research On The Mechanism Of AI Temperature-controlled Radiofrequency In The Treatment Of Vaginal Laxity

Objective: To investigate the mechanism of AI temperature-controlled radiofrequency technology in the treatment of vaginal laxity through randomized,controlled,and repeated animal model experiments,and to provide theoretical evidence for future radiofrequency treatment of vaginal laxity.Methods: Ten unlabored New Zealand White rabbits were the untreated group,and 30 New Zealand White rabbits with four consecutive deliveries were divided into the relaxation group,relaxation + sham treatment group,and relaxation + treatment group,(10 in the relaxation group,10 in relaxation + sham treatment group,and 10 in relaxation + treatment group)using the random number table method.The relaxation +sham treatment group was treated continuously for 5 sessions with the radiofrequency treatment instrument off,and the relaxation + treatment group was treated continuously for 5 sessions with the radiofrequency treatment instrument normal.After treatment,anterior vaginal wall tissues were taken and the m RNA expression levels of TIMP-1,COL-I,COL-III,TGF-β1 and MMP-1 in each group were detected by q PCR,and the protein expression levels of TIMP-1,COL-I,COL-III,TGF-β1 and MMP-1 in each group were detected by Western Blot.The experimental results were statistically analyzed using SPSS 26.0.p < 0.05 was considered a statistically significant difference.Results:1.In the untreated group compared with the relaxed group,the m RNA expression levels and protein expression of TIMP-1,COL-I,COL-III,and TGF-β1 were significantly decreased(P < 0.05)and the m RNA expression levels and protein expression of MMP-1 were significantly increased(P < 0.05)in the anterior vaginal wall tissues of New Zealand rabbits in the relaxed group,and the differences were statistically significant.2.In the relaxation group,the m RNA expression levels and protein expression of TIMP-1,COL-I,COL-III,and TGF-β1 were significantly increased(P < 0.05)and the m RNA expression levels and protein expression of MMP-1 were significantly decreased(P < 0.05)in the anterior vaginal wall tissues of New Zealand rabbits after 5 sessions of radiofrequency treatment,and the differences were statistically significant.3.In the relaxation group,the m RNA expression levels and protein expression of TIMP-1,COL-I,and TGF-β1 were significantly increased(P < 0.05)and the m RNA expression levels and protein expression of MMP-1 were significantly decreased(P <0.05)in the tissue of the anterior vaginal wall of New Zealand rabbits in the treatment group after 5 consecutive sessions of treatment with the radiofrequency treatment instrument turned off.),and the differences were statistically significant.the expression of COL-III was increased,but not statistically significant.Conclusion:1.Four consecutive deliveries of New Zealand White rabbits showed vaginal laxity,which can be used as an animal model to study vaginal laxity.2.Radiofrequency treatment significantly improved the symptoms of vaginal laxity in New Zealand White rabbits,and improved the symptoms of vaginal laxity by increasing the content of TIMP-1,COL-I,COL-III,and TGF-β1 and decreasing the content of MMP-1 in vaginal tissues.