The Foundation And Quality Standards Of Anti-inflammatory Substances Of Qingre Huadu Wan Were Ascend

As a classic prescription in China,Qingre Huadu Wan has a good effect of clearing heat and reducing poison,reducing swelling and reducing pain.It is often used to treat children’s body heat and irritability,sore throat,and residual poison after rash.It has a very good clinical curative effect on pediatric fever.Due to the recent years of children’s drug types and varieties are less.Therefore,it is particularly important to clarify the pharmacodynamic substances in the classical Chinese medicine prescriptions and control the relevant quality standards.In this study,UPLC-Q-TQF-MS/MS technology was used to establish the fingerprint of 29 peaks and identify the anti-inflammatory analysis method to analyze the anti-inflammatory drug effect components to provide the theoretical basis for the subsequent quality standard study.Combined with the results of spectral effect analysis,the quality standard of Qingre Huadu Wan was improved,the draft quality standard was formulated,and the stability was investigated.Methods: By preparing different batches of methanol extracts of 10 depyrochemical poison pills,the relevant fingerprints were established by high performance liquid chromatography(HPLC),and the relevant methodologies were investigated while confirming their common peaks.The HPLC-ESI-MS/MS analysis method was used to qualitatively characterize the relevant chemical components,and the molecular structure of the compound was derived according to the ion peak resolution mass spectrometry data of the corresponding molecule and the corresponding ion fragment information.The anti-inflammatory activity of different batches of extracts of Qingre Huadu Wan was determined by macrophage RAW264.7,and the anti-inflammatory activity of different batches of extracts of Qingre Huadu Wan was determined by Griess assay and ELISA assay,and the relationship between the common peaks of different batches of Qingre Huadu Wan and different inflammatory factors(IL-6,NO,TNF-αand IL-1β)was determined by gray correlation analysis method,and the contribution degree of different common peaks was derived according to the analysis results.According to the contribution degree of different common peaks,the quality standards of depyrochemical poison pills were improved,and the relevant content determination items and thin-layer examination chromatographic investigation were added,because it was a powder of Chinese medicinal materials,so the corresponding microscopic identification was added to determine its safety and controllability.Formulate the draft of its relevant quality standards,and conduct long-term stability studies on the characteristics,moisture,weight,microbial limit,thin layer,content determination and other aspects of the depyrochemical poison pill.Strive for comprehensive control of the deheating poison pill.Results: It was confirmed that there were 29 total peaks of HPLC,and 28 total peak chemical components and 1 unknown compound were derived and identified by HPLC-ESI-M S/MS analysis method.In vitro anti-inflammatory experiment,proved that it has good anti-inflammatory activity,using gray correlation analysis method,the analysis results show that29 total peak of RAW264.7 cells anti-inflammatory activity correlation are greater than 0.79,a variety of components together play anti-inflammatory effect,including forsythia,baicalin anti-inflammatory drug correlation are above 0.9,main contribution peak for anti-inflammatory activity.At the same time,on the basis of the original standard,increase moisture,weight difference check item,the porkahoe,forsythia,baicalensis,licorice,cinnabar,chrysanthemum microscopic differential study,and optimize the original thin layer identification study at the same time added forsythia,rhubarb,Scutellaria baicalensis,gentian thin layer chromatography identification,and methodology.According to the study of relevant anti-inflammatory active substances and the spectrum relationship and the guidance theory of traditional Chinese medicine,the two index components of baicalin in baicalensis and forsythia in forsythia were studied.The stability of the pill was studied for 6 months,and we tried to improve the quality control of the pill.