Study On The Expression Of FSHR And SIRT1 Gene Mutations In ART Female Granulosa Cells

Objective:Follicle Stimulating Hormone Receptor(FSHR)gene rs6166 and Silent Information Regulator l,by using gene sequencing technology.SIRT1 gene rs2236319 polymorphism in Assisted Reproductive Technology(Assisted Reproductive Technology,Expression of ART in female granulosa cells was associated with ovarian reserve function,pregnancy outcome,ovarian responsiveness,and ovarian hyperstimulation syndrome(OHSS),which provided a basis for individualized treatment of ART in assisted pregnancy.Methods:In this study,103 infertile women who received ART treatment in Reproductive Medicine Center of Affiliated Hospital of Inner Mongolia Medical University from September2020 to September 2022 were selected,and their general information was recorded,and follicle stimulating hormone was detected on the third day of menstruation.FSH levels,anti-mullerian hormone(AMH),vaginal B-ultrasonography were examined for follicle number in bilateral ovarian basal sinus,ovulation induction and embryonic development,clinical pregnancy rate,ovarian reactivity and OHSS incidence at the 2nd and 3rd days of menstrulation.Total RNA of granulosa cells was extracted from waste follicle fluid after egg extraction.c DNA was obtained by reverse transcription.The genotypes of FSHR-rs6166 and SIRT1-rs2236319 were determined by PCR amplification and gene sequencing.According to the results of gene sequencing of FSHR-rs6166 and SIRT1-rs2236319 polymorphic loci,they were divided into AA group(wild type)(n=51 cases),AG group(heterozygote type)(n=40cases)and GG group(mutant type)(n=12 cases).SIRT1-rs2236319 can be divided into: TT group(wild type)(n = 59 cases),the TC group(hybrid)(n = 35 cases)and CC group(mutant)(n = 9),the experimental results applied Haploview linkage disequilibrium analysis software,using analysis of variance and 2 inspection carries on the statistical analysis,The differences in general information,ovarian reserve function,pregnancy outcome,ovarian reactivity and incidence of OHSS among all groups were compared..result:1.Among the 103 ART women included,AA genotype and SIRT1-rs2236319 genotype were the most common among FSHR-rs6166 and SIRT1-rs2236319 genotypes,with frequencies of 49.5% and 57.3% respectively.2.The basal follicle stimulating hormone(b FSH)value in GG and AG groups was higher than that in AA group(P=0.003).The anti-Mullerian hormone(AMH)in GG and AG groups was lower than that in AA group(P=0.036).SIRT1-rs2236319 among the three groups: AMH in CC group was significantly lower than that in TT and TC groups(P=0.040),and egg number in CC group was significantly higher than that in TT and TC groups(P=0.023).3.The polymorphisms of FSHR-rs6166 and SIRT1-rs2236319 genes were not significantly correlated with ovarian responsiveness,the incidence of ovarian hyperstimulation syndrome(OHSS)and pregnancy outcome(P>0.05).Conclusion:FSHR-rs6166:1.GG group(mutant type)and AG group(mutant heterozygote type)may be associated with decreased ovarian reserve function.2.There was no correlation between the three genotypes and pregnancy outcome,ovarian responsiveness and OHSS incidence.SIRT1-rs2236319:1.CC group(mutant type)may be related to the decline of female ovarian reserve function;2.The number of oocytes obtained in CC group(mutant)was significantly higher than that in TT group and TC group,which accelerated the consumption of follicles and accelerated the decline of ovarian function;3.There was no correlation between the three genotypes and pregnancy outcome,ovarian responsiveness and OHSS incidence.