Related Metabolic Pathways In Patients With Multiple Sclerosis

Objective Through non-target metabolic analysis of peripheral blood of multiple sclerosis patients and normal controls,the differential metabolites in peripheral blood were screened,and the differential metabolites were analyzed to participate in the biological process and regulatory pathway of the disease,so as to reveal the pathogenesis of multiple sclerosis,promote our understanding of the pathogenesis of multiple sclerosis,and provide help for clinical diagnosis and differential diagnosis.Search for better therapeutic targets,so as to achieve early diagnosis,early intervention,and improve prognosis.Methods Venous blood samples were collected from 37 patients with multiple sclerosis as the case group,and venous blood samples from 29 healthy people without corresponding contraindications as the control group.This experiment was based on high-resolution untargeted metabolomics technology for detection,quality evaluation and data analysis.Ultra-high performance liquid chromatography-tandem time-of-flight mass spectrometry was used to detect the case group and the control group.The metabolites detected were structurally identified and the data were preprocessed to ensure the stability of the equipment,good experimental repeatability and reliability of data quality.Six quality control items including Total ion chromatogram(TIC)and Principal Component Analysis(PCA)were used for comprehensive evaluation.Univariate statistical analysis and multidimensional statistical analysis were used to analyze the differences between groups,and the differential metabolites were screened.Finally,bioinformatics analysis was performed on the differential metabolites and their related metabolic pathways.Results In this experiment,a total of 241 metabolites were identified after combining positive and negative ions,and lipids,lipid-like substances,and organic acids accounted for the highest proportion.21 differential metabolites were screened between the case group and the healthy control group,among which DL-lactate、myristic acid、1-Stearoyl-2-hydroxy-snglycero-3-phosphocholine、Hydrocortisone、1-Palmitoylglycerol、Alpha-N-PhenylacetylL-glutamine、1-Oleoyl-sn-glycero-3-phosphocholine、4-Pyridoxic acid、L-Palmitoylcarnitine、Betaine were up-regulated.It was down-regulated by embelin、sunitinib、N-Palmitoylsphingosine、palmitic acid、sphingomyelin(d18:1/18:0)、linoleic acid、cis-9-Palmitoleic acid、lathosterol、(3-Carboxypropyl)trimethylammonium cation 、 epsilon-Caprolactam 、 N1-Methyl-2-pyridone-5-carboxamide.The metabolic pathways involved in the above differential metabolites included fatty acid biosynthesis,fatty acid degradation,unsaturated fatty acid biosynthesis,pyruvate metabolism,aldosterone-regulated sodium reabsorption,necroptosis,etc.Conclusion There are significant differences in peripheral blood metabolites between patients with multiple sclerosis and healthy controls,among which fatty acids and lipids are the two most abundant differential metabolites.These differential metabolites have certain reference value for finding biomarkers of the disease.Fatty acid biosynthesis,fatty acid degradation,unsaturated fatty acid biosynthesis,pyruvate metabolism,aldosterone-regulated sodium reabsorption,necroptosis and other metabolic pathways may be related to the pathogenesis of multiple sclerosis.The study of metabolites and metabolic pathways in multiple sclerosis enables us to further understand the pathophysiological process of the disease,promotes the understanding of the pathogenesis,and the intake of some polyunsaturated fatty acids is beneficial to the occurrence and development of the disease,which provides certain help and clues for clinical diagnosis and treatment.