| Objective:This study aims to detect the serum levels of LXA4,Ang-2,and ENS in patients with endometriosis and dysmenorrhea.We aim to analyze the correlation between these three cytokines and R-AFS staging and dysmenorrhea,which will provide a unique perspective on predicting the disease development of endometriosis patients and suggesting new ideas for treatment.Methods:A total of 130 patients with endometriosis diagnosed by laparoscopy and pathology admitted to Weifang People’s Hospital and Shouguang Maternal and Child Health Hospital from December 2019 to December 2021 were selected.120 patients diagnosed with benign ovarian tumor and no dysmenorrhea via laparoscopy and pathology in the same period were chosen as the control group.Among the 130 patients with endometriosis who met the inclusion criteria,73 cases were categorized as the mild group,and 57 cases were placed in the moderate severe group.The patients diagnosed with endometriosis were classified into two groups based on the R-AFS staging system: the first group comprised stage I and II patients,while the second group consisted of stage III and IV patients.The observation group had 71 patients in the stage III-IV group and 59 patients in the stage I-II group.After admission,the patients’ general information was collected,including their age,menstrual cycle,course of disease,menarche age,number of pregnancies,number of births,height,and weight.Dysmenorrhea VAS scores were obtained.During laparoscopic examination,the ectopic tissue position,size,extent of adhesion,and closure of rectal and uterine lacunae were recorded for the observation group,which was used to determine the R-AFS staging.The double antibody sandwich enzyme-linked immunosorbent assay(ELISA)was used to determine the serum levels of Lx A4,Ang-2,and ENS.SPSS 22.0 software was employed to conduct statistical analysis,with the independent sample t-test used for measurement data,the chi-square test used for counting data comparison,and the groups with non-normally distributed data were analyzed with Wilcoxon or Kruskal Wallis rank sum test.We conducted correlation analysis with Spearman’s correlation analysis.The statistical significance level was set at P < 0.05.No significant difference was found at P > 0.05.Results:(1)No significant differences were revealed in age,menstrual cycle,menarche age,course of disease,or other general data between the control group and the observation group(P > 0.05),indicating comparability.(2)Serum levels of Lx A4,Ang-2,and ENs were compared between the observation group and the control group,and the comparison between the two groups was statistically significant(P < 0.05).The serum levels of Lx A4 in the observation group were lower than those in the control group.The serum level of Ang-2 was significantly higher in the observation group than in the control group(P < 0.05).Similarly,the serum level of ENs showed the similar trends(P < 0.05).(3)When comparing Lx A4,Ang-2,and ENs in different degrees of dysmenorrhea,the serum Lx A4 levels in the mild and moderate-severe groups were statistically significant(P < 0.05),and the serum Lx A4 levels in the mild group were higher than those in the moderate-severe group.The serum level of Ang-2 in the mild group was significantly lower than that in the moderate and severe groups(P < 0.05),and the serum level of ENs in the mild group was also significantly lower than that in the moderate and severe groups(P < 0.05).(4)Comparison of Lx A4,Ang-2,and ENs in different R-AFS stages showed that the level of serum Lx A4 in stage I-II was significantly lower than that in stage IV(P < 0.05).Similarly,the level of serum Ang-2 in stage I-II was significantly lower than that in stage IV(P < 0.05).Moreover,the level of serum ENs in stage I-II was significantly higher than that in stage IV(P < 0.05).(5)Correlation analysis of Lx A4,Ang-2,and ENs with the degree of dysmenorrhea and R-AFS staging showed that Lx A4 was negatively correlated with both the degree of dysmenorrhea and R-AFS staging in the observation group(r =-0.918;P < 0.001),indicating that higher levels of Lx A4 were associated with less severe dysmenorrhea and lower R-AFS staging.Similarly,serum Ang-2 level was positively correlated with both the degree of dysmenorrhea(r = 0.647;P < 0.001)and R-AFS stage(r = 0.531;P < 0.001),indicating that higher levels of Ang-2 were associated with more severe dysmenorrhea and higher R-AFS staging.Additionally,serum ENS level was positively correlated with both the degree of dysmenorrhea(r = 0.712;P < 0.001)and R-AFS stage(r = 0.639;P <0.001),indicating that higher levels of ENS were associated with more severe dysmenorrhea and higher R-AFS staging.Conclusion:(1)The expression of LXA4 was found to be low in EMS patients,and its serum level decreased with increasing severity of dysmenorrhea and higher R-AFS stage.A negative association was revealed between LXA4 and R-AFS stage and dysmenorrhea degree by correlation analysis.(2)Ang-2 expression was found to be elevated in EMS patients,and its serum level increased with increasing severity of dysmenorrhea and higher R-AFS stage.Correlation analysis showed a positive association between Ang-2 and R-AFS stage and dysmenorrhea degree.(3)ENS expression was found to be high in EMS patients,and its serum level increased with increasing severity of dysmenorrhea and higher R-AFS stage.A positive association was revealed between ENS and R-AFS stage and dysmenorrhea degree by correlation analysis.(4)The levels of LXA4,Ang-2,and ENS can reflect the severity and dysmenorrhea of EMS patients,which have the potential to provide guidance for clinical understanding of the degree of disease development and subsequent treatment. |
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