Effects Of Sleeve Gastrectomy On Blood Glucose And Bile Acid In Obese Rats With Type 2 Diabetes Mellitus

Objective:To investigate the effects of sleeve gastrectomy on blood glucose and bile acid in obese rats with type 2 diabetes mellitus.Methods:The obese combined with type 2 diabetes mellitus rat model was successfully constructed by intraperitoneal injection of STZ solution in 8-week-old wistar rats after 6weeks of high-fat diet feeding.To ensure the objectivity and reliability of the experimental results,we used the random grouping principle to group the experiments,including the blank control group（C group,n=10）,sham operation group（Sham group,n=12）,and SG group（n=12）.After 1 week of high-fat diet feeding,sleeve gastrectomy and sham operation were performed.The changes of body weight and food intake of rats were observed before and 1,2,4,6 and 8 weeks after surgery,and the changes of fasting blood glucose were measured by blood sampling before and 1,2,4 and 8 weeks after surgery,and the changes of insulin were measured by blood sampling at 2,4 and 8 weeks after surgery.Glucose tolerance test was performed before and at 2 and 8 weeks after surgery,and insulin tolerance test was performed before and at 8 weeks after surgery.Blood was collected before and 8 weeks after surgery to measure the change of GLP-1 and the change of TBA.Results:（1）A total of 45 rats participated in the experiment,among which 38 rats fit the model of obesity combined with type 2 diabetes mellitus.5 rats in the SG group died within 1 week after surgery,1 rat died in the sham surgery group,and the rest survived until the end of the experiment.（2）The body weight of rats in the SG group decreased significantly（P<0.05）compared with that before surgery at 1 week and 2 weeks postoperatively,and gradually increased（P<0.05）from 4 weeks to 8 weeks postoperatively compared with the first 2weeks.From 1 week to 8 weeks postoperatively,the difference in body weight between the three groups was statistically significant（P<0.05）.From 1 week to 8 weeks after surgery,the amount of food eaten by rats in the SG group decreased significantly compared with that before surgery（P<0.05）,and there was a statistically significant difference between the amount of food eaten by rats in the three groups（P<0.05）.（3）From 1 week to 8 weeks after surgery,the fasting blood glucose levels of rats in the SG group decreased significantly（P<0.05）compared with those before surgery,and the fasting blood glucose levels of rats in the three groups differed significantly（P<0.05）.From 2 weeks to 8 weeks after surgery,the fasting insulin levels of rats in the SG group decreased significantly compared with those before surgery（P<0.05）,and the differences in fasting insulin levels of rats in the three groups were significant（P<0.05）.From 2 weeks to 8 weeks after surgery,the HOMA-IR of rats in the SG group decreased significantly compared with that before surgery（P<0.05）,and the difference between the HOMA-IR of rats in the three groups was significant（P<0.05）.（4）At 2 weeks postoperatively,the area under the glucose tolerance curve(AUC_IPGTT)of rats in the SG group decreased significantly compared with that before surgery（P<0.05）.At 2 weeks and 8 weeks postoperatively,the AUC_IPGTTof rats in the three groups differed significantly（P<0.05）.At 8 weeks postoperatively,the area under the insulin tolerance curve(AUC_ITT)of rats in the SG group decreased significantly compared with that before surgery（P<0.05）,and the difference between the AUC_ITTof rats in the three groups was statistically significant（P<0.05）.（5）At 2 weeks and 8 weeks after surgery,GLP-1 was significantly higher in the SG group than before surgery（P<0.05）,and the difference between the three groups of rats was statistically significant（P<0.05）.At 2 weeks and 8 weeks after surgery,the TBA of rats in the SG group was significantly higher than before（P<0.05）,and the difference between the three groups was statistically significant（P<0.05）.Conclusion:Sleeve gastrectomy reduced body weight,decreased fasting glucose,reduced insulin levels,improved insulin resistance,and promoted GLP-1 and bile acid secretion in obese rats with combined type 2 diabetes.