Intestinal Microecological Characteristics Of Different Intestinal Types In Patients With Functional Constipation

Objective:To investigate the bacterial composition,SCFAs levels,and methane content of different intestinal types in patients with functional constipation.Methods:A total of 49 patients with functional constipation,who were diagnosed and treated in the Third Hospital of Shanxi Medical University from May 2022 to February 2023,were selected to collect personal information and feces,and to conduct quantitative real-time PCR(q PCR)to detect the diversity of intestinal type,flora structure,and genus level microbial composition,revealing the characteristics and differences of intestinal microflora in each group.The expression level of short chain fatty acids(SCFAs)in feces was detected by gas chromatography,and the methane content in the in vitro model after fermentation for 24 hours was detected by intestinal fermentation gas analyzer.Finally,the intestinal microecological characteristics of two different intestinal types of patients were analyzed and compared using statistical methods.Results:1.Compared with the Bacteroides group,the propionic acid and butyric acid content in the Prevotella group were significantly decreased(0.048,0.044,p<0.05),while the acetic acid content,valeric acid content,and methane content were not significantly different.2.The q PCR detection showed that the content of Lactobacillus and Bifidobacterium in the Bacteroides group was mostly less than 60%,while the content of butyric-acid bacteria was mostly less than 50%.The content of Lactobacillus in the Prevotella group is mostly lower than 55%,while the content of butyric-acid bacteria is mostly lower than 50%.3.Among the two beneficial bacteria in the intestine,Lactobacillus has a significant correlation with acetic acid,propionic acid,butyric acid,and valeric acid,while Bifidobacterium only has a positive correlation with propionic acid,butyric acid,and valeric acid.Conclusion:1.The composition of beneficial and harmful bacteria in the feces of patients in the Bacteroides group and the Prevotella group was similar.2.The Bacteroides group can more effectively decompose carbohydrates and produce corresponding SCFAs,resulting in higher propionic acid and butyric acid content than the Prevotella group.