The Effect Of Polyoxometalate On Proliferation Of Cervical Cancer Cell Study

Objective:A novel Polyoxometalate（POM）Na₇PW₁₁O₃₉ was used to treat Si Ha cells of cervical cancer.The effects of Na₇PW₁₁O₃₉ on the proliferation of Si Ha cells were observed and the possible mechanism of action was discussed.Methods:It was selected as tumor cell models to cervical cancer Si Ha cells,and the antitumor activity of the newly synthesized Na₇PW₁₁O₃₉ was screened according to the in vitro screening method of anti-tumor compounds of the NCI.Na₇PW₁₁O₃₉ solution of different concentrations were prepared according to the method of multiple ratio dilution,and its effect on tumor cells was observed and counted.Cisplatin solution was selected as positive control group,and corresponding volume culture medium was added as blank control group.The toxic effect of Na₇PW₁₁O₃₉ on Si Ha cells of cervical cancer was determined by CCK8,and its IC₅₀ and inhibitory rate of Si Ha cell proliferation of the substance were calculated.The effect of Na₇PW₁₁O₃₉ on Si Ha cell cycle of cervical cancer was detected by flow cytometry.The effect of Na₇PW₁₁O₃₉ on apoptosis of Si Ha cells of cervical cancer was determined by flow cytometry with Annexin V-FITC/PI fluorescence staining.The effect of Na₇PW₁₁O₃₉ on the transcription and expression level of HPV16 E6 m RNA was detected by RT-PCR.Finally,Western Blot was used to detect the effect of Na₇PW₁₁O₃₉ on HPV16 E6 protein expression.Results:This study confirmed the Na₇PW₁₁O₃₉ has inhibitory effect on cervical cancer Si Ha cells,there was no statistically significant difference compared with cisplatin,and calculate the 24h,48h,72h IC₅₀ were 419.6,359.5,212.3μmol/L,the proliferation inhibition rate increases over time,Further by flow cytometry instrument to detect Na₇PW₁₁O₃₉ in 0,212.3,359.5,419.6μmol/L concentration,at G0 cell respectively17.4%,15.7%,5.84%,6.4%,p=0.03;The cells in G1 phase accounted for 20.4%,15%,1.86%and 0%,respectively,p=0.158.The cells in G2/M phase accounted for 16.3%,23.6%,57.9%and 66%,respectively,p=0.045.S phase cells accounted for 45.9%,45.7%,34.4%and 6.4%,respectively,p=0.000.Flow cytometry instrument to detect Na₇PW₁₁O₃₉ in 0,212.3,359.5,419.6μmol/L concentration,the apoptotic cells were accounted for 8.9%of total cells,17.8%,36.7%,42.5%,p=0.043).RT-PCR showed that the HPV16 E6 m RNA expression level decreased under the influence of Na₇PW₁₁O₃₉,and Western Blot indicated that the expression of HPV16 E6 protein in cervical cancer Si Ha cells decreased under the influence of Na₇PW₁₁O₃₉.Conclusion:1.Na₇PW₁₁O₃₉ has an inhibitory effect on Si Ha cells of cervical cancer,and 24h,48h and 72h IC50 were 419.6,359.5,212.3μmol/L,and the proliferation inhibition rate increased with time and dosage.2.Flow cytometry showed that Na₇PW₁₁O₃₉ blocked the G2/M phase of the cell cycle,thus affecting cell proliferation.3.Flow cytometry instrument to detect Na₇PW₁₁O₃₉ Si Ha cells apoptosis,and found419.6μmol/L on the Si Ha cell apoptosis is the most obvious.4.RT-PCR showed that HPV16 E6 m RNA expression level decreased under the effect of Na₇PW₁₁O₃₉.5.Western Blot indicated that Na₇PW₁₁O₃₉ inhibited the expression of HPV16 E6protein in cervical cancer Si Ha cells.