Establishment Of A Selective Liver Lobe Tumor-bearing Mouse Model Of ALPPS And Study Of ALPPS Effect On Liver Regeneration

Background: Hepatectomy is one of the most commonly used procedures in hepatobiliary surgery,and its indications include hepatocellular carcinoma,intrahepatic cholangiocarcinoma,liver metastatic tumor and many other diseases.However,postoperative residual liver deficiency greatly limits the application of hepatectomy.Therefore,improving liver function reserve is the primary problem to improve the success rate of surgical resection of large liver tumors.Associating liver partition and portal vein ligation for staged hepatectomy(ALPPS)can shorten the surgical interval from 5 to 10 weeks to 7 to 13 days,which brings new hope for the treatment of large liver tumors.However,the mechanism of rapid hepatocyte proliferation caused by ALPPS surgery has not been clarified.At the same time,there is controversy about the effect of ALPPS on liver tumors after surgery.Therefore,the establishment of a reliable animal model of ALPPS bearing tumor is a necessary condition for further improvement of ALPPS related studies.Purpose: A selective liver tumor bearing model close to the physiological characteristics of human liver tumor was constructed,and on this basis,a selective liver tumor bearing ALPPS model was constructed,and the influence of ALPSS on liver tumor and liver regeneration was initially explored,which laid a solid foundation and reference for further promoting ALPPS related research and clinical application.Method: BALB/C mice were inoculated with CT26 colon cancer cell line transfected with GFP virus via spleen.Fluorescence microscopy was used to observe the formation of liver lobe tumor in mice under different times of spleen ligation and portal vein occlusion,and serum detection was used to compare the postoperative changes of liver function.The optimal time of spleen ligation and portal vein occlusion was used to construct the selective tumor bearing model of mice in the middle and right lobe of the liver,and the ALPPS mouse model was constructed on this basis.The effects of ALPPS operation on tumor and normal liver tissue regeneration were analyzed by weighing each liver lobe of mice.Results: By ligation and excision of the spleen 1 min after injection of CT26 cells,and re-opening the blocked portal vein branch 1 min after splenectomy,an ideal selective tumor bearing mouse model can be established with little effect on liver function.Based on the selective liver leaf tumor bearing model,the selective liver leaf tumor bearing ALPPS model can be constructed by ligation of the portal vein branch of the tumor bearing leaf and disassembling the normal liver and the tumor liver leaf.Conclusion: The spleen was lapped and resected 1 min after the injection of CT26 into the spleen,and the microvascular forceps and microsutures were released 1min after the splenectomy.Then the ideal model of ALLPS mice with selective liver lobe tumor was constructed by ligation of the portal vein in the middle and right lobe of the liver and the liver parenchyma in the middle and left lobe of the liver.The ALPPS procedure inhibits the growth of liver tumors and reduces the time required for residual liver regeneration to the desired size.