Surface Acoustic Wave CA153 Immunosensor Based On Au-TiO₂/AlN

Tumor biomarkers are substances produced and released by tumor cells,mainly present in patients’body fluids,and have important indicative significance for early prevention,clinical detection,and postoperative recovery of tumors.As a tumor marker of breast cancer,CA153 is an important reference index for early prevention and postoperative rehabilitation detection of breast cancer.Breast cancer,as one of the most common cancers among women,seriously threatens women’s physical health.In recent years,with the change of people’s diet and living habits,the incidence rate of breast cancer in China has increased year by year.Therefore,there is an urgent need for a convenient,accurate,and sensitive immunosensor for real-time detection of tumor markers.This article presents a surface acoustic wave immunosensor based on Au-TiO₂/Al N for the detection of tumor marker CA153.In the experiment,the delay linear SAW device was selected.Through Magnetron sputtering technology,dense Al N films were grown on the device surface.PDMS gel was used to prepare a micro test room in the delay zone of the device.The Au-TiO₂ composite films were grown in the delay zone of the device by thermal reduction method.CA153 antigen was detected by incubating CA153 antibody and bovine serum albumin.Enhance the response of surface acoustic wave immunosensors by optimizing sensitive membranes,antibody incubation time,antibody incubation concentration,and antigen incubation time.By conducting antigen concentration testing on the optimized immune sensor,the sensitivity of the sensor was-0.01643 MHz(ln(m U m L^-1))^-1.The linear equation between the frequency shift of the sensor and the antigen concentration was F=-0.04284-0.01643 ln[CA153](MHz,m U m L^-1),with a linear correlation of R=0.98482.The linear range for antigen concentration detection was 0.1 m U m L^-1-100 m U m L^-1,and the lower limit of antigen concentration detection（LOD）was 0.07372 m U m L^-1.The reproducibility of the sensor was also evaluated The reproducibility,stability,and specificity tests confirm that the surface acoustic wave immunosensor prepared in this experiment has significant reference value in the field of clinical medicine.