Changes Of Mitochondrial Damage Index And Cytokine Levels Of T Cells After Craniocerebral Injury

Objective: This article uses flow cytometry to dynamically detect the mitochondrial damage and cytokine levels of serum T lymphocytes and their subsets in patients with traumatic brain injury(TBI),so as to gain a deeper understanding of their role in mild,moderate and severe traumatic brain injury,and the changes in each period after craniocerebral injury provide a reference for TBI patients’ condition judgment and prognosis evaluation.Methods: From April 22,2021 to July 2,2021,70 TBI patients admitted to Shaoxing Central Hospital were collected,and the patients were divided into mild group,moderate group and severe group according to Glasgow Coma Scale(GCS)group,and a normal control group.On the 1st day,6th day and 11 th day after injury,peripheral venous blood was drawn,and flow cytometry was used to detect the changes in mitochondrial damage and cytokine levels of T lymphocyte subsets in serum.Three months after discharge,the patients were assessed and recorded using Glasgow Outcome Scale(GOS).Results: After the occurrence of TBI,the number of CD3+,CD4+ and CD8+ T lymphocytes in the peripheral blood of patients will decrease sharply,but then there will be a trend of gradual recovery.One day is still below normal.The mitochondrial damage index of T lymphocytes will rise rapidly after injury,reach the highest value on the first day after injury,and then gradually decline,presenting a "single peak" curve.Normal level is high.The mitochondrial damage index of CD3+,CD4+ and CD8+ T lymphocytes measured on the first day of admission was positively correlated with the severity of the patient’s condition and negatively correlated with the prognosis of the patient.After the occurrence of TBI,the concentrations of interleukin(Interleukin,IL)-4,IL-6,tumor necrosis factor α(Tumornecroticfactor α,TNF-α),and interferon γ(Interferon γ,IFN-γ)in the serum of patients all increased significantly,where the concentration of IL-6 was positively correlated with the severity of the patient’s condition.The concentration of IL-6 in the serum of TBI patients rose rapidly after injury,reached the highest value on the first day after injury,and then gradually decreased,presenting a "single peak" curve.higher than normal.The level of IL-6measured on the first day of admission in patients with moderate to severe TBI was negatively correlated with the prognosis of the patients.Conclusion: The mitochondrial damage index and IL-6 of T lymphocytes and their subsets can be used as an important indicator to effectively evaluate the prognosis of TBI patients and judge the severity of the disease,and it is worthy of wide application in clinical practice.