Study The Medication Law And Mechanism Of BuShenTongLuo Method In The Treatment Of Diabetic Kidney Disease Based On The TCM Inheritance Support System And Network Pharmacology

Objective To explore the medication rules and mechanisms of Bu Shen Tong Luo Method for diabetic kidney disease,and provide data support for clinical application and promotion.Methods1.Collect modern medical literature on the treatment of diabetic nephropathy by kidney onic method from the establishment of the library to August 2022 in China Journals Full-text Database(CNKI),Wanfang Data Knowledge Service Platform,Weipu Information Chinese Science and Technology Journals Database(VIP)and other databases,and enter the literature that meets the inclusion criteria into the TCM inheritance support system,and conduct statistical analysis of the frequency of use of prescription drugs,four qi and five flavors,and menstruation.At the same time,the association rules and unsupervised hierarchical clustering algorithm of the software were used to extract the association rules,prescription analysis and new analysis between drugs,and the core drug groups of the treatment of diabetic nephropathy by kidney tonic communication method were obtained.2.The active ingredients and potential targets of the core drug group were screened by TCMSP and TCMID literature,the diabetic nephropathy targets were screened by OMIM,Drugbank and TTD databases,the common targets of the core drug group and diabetic nephropathy were obtained by using Venny2.1.0 software,and the "drug-component-target" network was established by using Cytoscape3.9.2 software.Protein interaction network analysis of common targets through String database;GO function enrichment analysis and KEGG signal pathway analysis of common targets were performed with the help of Metascape database,and microbiotic tools were used to visualize them.Finally,the core targets will be further screened under the condition of ≥ 2 times the median Dgree value,and the molecular docking verification of the core targets and their corresponding active ingredients will be carried out by Auto Dock.Results1.Included literature analysis: CNKI searched a total of 1404 articles,77 Wanfang,25 Weipu,and finally obtained a total of 120 articles after screening,and extracted 98 prescriptions.2.Medication statistics: a total of 98 prescriptions were entered,and the top ten flavored drugs with high frequency of use were used: astragalus 88 times,danshen 56 times,leech 53 times,dogwood 48 times,yam 47 times,rhubarb 45 times,raw ground yellow 41 times,poria40 times,ze diarrhea 35 times,goji berry 28 times;Four qi with warm drugs up to 387 times,cold 382,subflat 366 times;The five flavors are sweetened up to 680 times,bitter 458 times and spicy 257 times;The highest frequency of use was liver meridian 687 times,kidney meridian 510 times,and spleen meridian 499 times;The support degree was set to 30,the confidence level was 0.85,and the cumulative frequency of drug pairs was Danshen-Astragalus(51 times)and Leech-Astragalus(46 times),and 9 association rules were obtained,among which the association rules with high confidence were "Raw Rehmania->Astragalus","Yam-> Astragalus",and "Danshen->Astragalus".Set the correlation degree to 5and the penalty degree to 2,and four potential new party combinations can be evolved based on the unsupervised entropy hierarchical clustering algorithm.3.Network pharmacology: 142 active ingredients of 9 core drugs by kidney tonic method,601 active ingredient targets,1079 DKD disease targets,174 intersection targets of drugs and diseases,782 biological processes,22 cell components,47 molecular functions,and 135 related signaling pathways were obtained by enrichment analysis.Among them,the main active ingredients of kidney tonic tonification method in the treatment of diabetic nephropathy are quercetin,spinosangetin,isorhamnetin and kaempferol,etc.,the key targets are AKT1,IL-6,VEGFA,TNF,IL-1 β,GAPDH,and the main biological pathways are lipid and atherosclerosis,fluid shear stress and atherosclerosis,HIF-1,Th17 cell differentiation,insulin resistance,etc.The results of molecular docking showed that AKT1 had the strongest binding activity with isorhamnetin,GAPDH and quercetin,TNF and 7-O-methylisoconvex sadiol,IL-1β with kaempferol,and IL-1β with formononetin.Conclusion1.According to data mining and association analysis,the core formulas were obtained:astragalus,danshen,rhubarb,poria,yam,zeppelin,leech,raw ground yellow,peony peel.2.Through network pharmacology research,it is concluded that the core formula of Jiuwei drug treatment for diabetic nephropathy has the characteristics of multi-component,multi-target and multi-pathway.The core drug group may be through quercetin,kaempferol,formononetin,isorhamnetin,7-O-methylisoconvex sadiol,etc.to target AKT1,IL-6,VEGFA,TNF,IL-1β,GAPDH and other targets,and achieve kidney protection by regulating lipids and atherosclerosis,HIF-1,Th17,insulin resistance signaling pathways,etc.to regulate cell function and reduce vascular inflammatory response.