Research On The Role Of Glutaminergic Neurons In Working Memory Impairment Of Mice Induced By Microwave Radiation

With the increasing application of electromagnetic equipment in communication,agriculture,industry and military fields,the influence of electromagnetic radiation on human physical and mental health has been widely concerned.The brain is one of the most sensitive target organs of microwave radiation,and working memory as a higher cognitive function may be affected by microwave exposure.Glutamate,as a major excitatory neurotransmitter,is widely distributed in the brain,and plays important roles in learning and memory by combining the postsynaptic membrane through ionic glutamate receptors.In this study,the effects of microwave radiation on working memory and glutaminergic neurotransmission in related brain regions were investigated to provide a basis for clarifying the biological effects of microwave radiation damage and lay a theoretical foundation for radiation protection and intervention of people exposed to microwave.This thesis includes three studies,in study 1,146 C57BL/6N mice were divided into control group and radiation group.The mouse model of microwave radiation was established,and the effects of microwave radiation on cognitive function,tissue structure of related brain regions and neuronal activity were examined at different time points after radiation by behavioral methods such as Y maze and Morris water maze,pathological techniques such as hematoxylin-eosin,multichannel electrophysiology of in vitro brain slices and immunofluorescence.In study 2,30 C57BL/6N mice were divided into control group and radiation group.And fiber optic recording was used to detect the activity of calcium ions in ventral hippocampus and medial prefrontal cortex of mice after radiation.Immunofluorescence was used to detect the expression changes of glutamate receptors.In Study 3,34 C57BL/6N mice were divided into control group(group C),photoactivated group(R+Ch R2 group)and activated control virus group(R+EGFP group).The role of glutaminergic neurons in microwave radiation induced working memory impairment was detected by optogenetics.The results showed that:(1)microwave radiation reduced the spontaneous alternations of Y-maze,damaged the neurons of hippocampus and prefrontal cortex,destroyed the plasticity of synaptic structure,decreased the electrical activity of hippocampal neurons,and reduced the expression of c-fos in ventral hippocampus and medial prefrontal cortex.(2)Microwave radiation reduced the area under the calcium ion activity curve of glutaminergic neurons in v HPC and m PFC of mice,and decreased the expression of Glu N1 and Glu N2B receptor.(3)Activation of glutaminergic neurons in v HPC alleviated the decrease of spontaneous alternation of Y maze induced by microwave radiation.In conclusion,30m W/cm~2 microwave exposure could induce the impairment of working memory,injuries of the structure and function of the hippocampus and prefrontal cortex,the decrease of the neurons activities of hippocampus in vitro,and the activity of glutaminergic neurons in the v HPC and m PFC,and the downregulation of the expression of glutamate receptor subunits.Glutamate neurons in ventral hippocampus played an important role in microwave-induced impairment of working memory in mice.