Effects Of Salt Stress On The Ultrastructure And Secondary Metabolites Of Mycorrhizal Elaeagnus Angustifolia Leave

Salt stress is a common environmental factor in nature,which restricts the growth of plants and hinders the development of agricultural production.Arbuscular mycorrhizal fungi(AMF)can establish symbiotic relationships with plants and improve plant stress resistance.Elaeagnus angustifolia is a salt-tolerant woody plant,which can form symbiosis with AMF.In order to explore the Photosynthetic Physiological Indexes of mycorrhizal Elaeagnus angustifolia seedlings and the response of leaf cell ultrastructure to salt stress.This study adopts the method of potted,the inoculation of AMF-Rhizophagus irregularis(M)and unvaccinated AMF(NM)narrow-leaved oleaster seedling,at 0 and 300 m M under Na Cl stress ultrastructure of leaf photosynthetic parameters,leaf,leaf blade secondary metabolites of flavonoids substances(rutin,quercetin,rhizoma kaempferiae phenol,waxberry,rat Li Su)was the content of indole acetic acid and salicylic acid content was determined by analysis.The results show that:(1)Pn,Tr,Gs and Ci of Elaeagnus angustifolia leaves treated with M were significantly higher than those treated with NM(p < 0.05).Significant decreases in Pn,Tr,Gs,and Ci were found in the leaves under the salt stress of M and NM,while M treatment had significantly reduced the influences from salt stress compared with those from NM(p < 0.05).(2)The cell structure of Elaeagnus angustifolia leaves treated with M and NM was damaged after salt stress,but the chloroplast structure of M treatment was more complete than that of NM treatment,and the number of plastid globules increased.(3)The content of flavonoids in leaves of Elaeagnus angustifolia seedlings treated with M and NM decreased significantly under salt stress,but the content of flavonoids in leaves of Elaeagnus angustifolia seedlings treated with M was still significantly higher than that treated with NM(p < 0.05),and the change extent of M treatment was significantly smaller than that of NM treatment(p < 0.05).(4)The contents of indoleacetic acid and salicylic acid in leaves of Elaeagnus angustifolia seedlings treated with M and NM decreased significantly under salt stress,but the contents of indoleacetic acid and salicylic acid in leaves of Elaeagnus angustifolia seedlings treated with M were still significantly higher than those treated with NM(p < 0.05),and the change range of M treatment was significantly smaller than that of NM treatment(p < 0.05).Our results further show that AMF can improve salt tolerance by affecting photosynthesis,ultrastructure of plant leaves and regulating secondary metabolic reactions of plant leaves.The experimental results enrich the salt tolerance of AM fungi and plants,and provide a theoretical basis for the improvement of saline-alkali land by the symbiosis of halophytes and AMF.