A Preliminary Study On The Growth And Drought Tolerance Of Arabidopsis Thaliana Expressing The Sugar Beet BvNLP2-like Gen

Sugar beet is a sugar crop planted in the north of China,and although research on sugar beet has not stopped in China for many years,genetic resources are still relatively poorly studied in China.NLP(NIN-like protein or nodulin-like protein)genes are widely present in living organisms,and existing studies have found that NLP genes are involved in the regulation of nitrate signaling and are closely related to plant growth and development and stress response.The existing studies found that NLP genes are involved in the regulation of nitrate signaling and are closely related to plant growth and development and stress response,and there are some differences in the types and numbers of NLP genes in different plants.In the early stage,the research group obtained a gene locus from the sugar beet genome and used the gene locus as the starting material to obtain the complete sequence of the coding region with a length of 2796 bp by gene cloning,and the main structural domain of the protein encoded by the gene was found to be consistent with the NLP gene family by bioinformatics analysis,so the gene was named Bv NLP2-like gene.In this study,we constructed the sugar beet BvNLP2-like gene overexpression vector p FGC5941-Bv NLP2,the gene knockout vector p HK2-Cas9-U6-BV and the gene knockout vector p HK2-Cas9-U6-AT for the homologous gene At RWP-RK in Arabidopsis thaliana,and investigated the effects of p FGC5941-Bv NLP2 and p HK2-Cas9-U6-AT transgenic Arabidopsis trait performance revealed that overexpression of the beet Bv NLP2-like gene promoted the growth of the below-ground part of Arabidopsis,and knockout of the Arabidopsis homolog At RWP-RK inhibited the growth of the below-ground part of Arabidopsis,and similar differences were observed in the growth of the above-ground part.The drought tolerance of the overexpressed Bv NLP2-like transgenic Arabidopsis seedlings was significantly enhanced and that of the knockout At RWP-RK transgenic seedlings was significantly decreased under mannitol simulated drought environment,and the growth of the overexpressed Bv NLP2-like transgenic Arabidopsis plants was significantly better than that of the knockout At RWP-RK transgenic plants.This indicates that overexpression of the sugar beet Bv NLP2-like gene positively regulates both growth and drought tolerance in Arabidopsis,while knockdown of the At RWP-RK gene inversely regulates the above traits.Based on the above results,it can be tentatively inferred that the Bv NLP2-like gene probably plays a role in promoting plant growth and development and drought stress tolerance,which provides a direction for studying the function of the Bv NLP2-like gene in sugar beet and lays the foundation for the study of the relationship between NLP genes and growth and development and stress tolerance.