Screening Of Inorganic Phosphate-degrading Bacteria In The Rhizosphere Of Paris Polyphylla And Its Inoculation Effec

Paris polyphylla var.yunnanensis is a perennial herbaceous plant of Liliaceae.As a genuine medicinal material in southwest China,it has significant medicinal value in anti-tumor and immune regulation.In natural conditions,the effective utilization of phosphorus in the sail of wild Paris polyphylla var.Yunnanensis is poor,resulting in a low reproduction rate,and the natural growth of rhizomes of medicinal parts is greatly retarded.Therefore,chemical fertilizer is applied in the artificial planting process to ensure its growth and development.However,the application of chemical fertilizer will lead to the risk of soil salinization.Based on this,this study improved the utilization rate of soil phosphorus by inoculating inorganic phosphorus hydrolysis bacteria in the process of artificial cultivation of Paris polyphylla var.yunnanensis to reduce the use of chemical fertilizer and non-point source pollution to soil.Through pot experiment at room temperature,by adding three inorganic phosphorus hydrolysis strains isolated from the rhizosphere soil of paris polyphylla var at an early stage,the effects of these inorganic phosphorus hydrolysis strains on growth,soil microenvironment,and medicinal quality of four-year-old paris polyphylla var are discussed.The results are as follows:Compared with the control group,the photosynthetic pigment content in the leaves of paris polypbylla was significantly increased by applying different demineralysis phosphorus strains,which promoted the growth and development of Paris polyphylla var.yunnanensis(Franch.)Hand.-Mazz.The activities of SOD,POD and CAT enzymes in the leaves of paris polypbylla promoted the accumulation of soluble protein and soluble sugar,reduced the content of malondialdehyde,and enhanced the stress resistance of paris polypbylla.The content and nutrient indexes of available nitrogen,available potassium and available phosphorus in rhizosphere soil were increased,causing the soil to transform from "fungal type" to "bacterial type".The soil microenvironment and its fertility were improved,and the absorption of soil phosphorus was also promoted.It enhanced the enrichment ability of nutrient elements and effectively improved the utilization rate of soil elements.The rhizosphere soil of Dipterygophyllum dipterygophyllum was slightly polluted after inoculation with inorganic bacteria,but the content of heavy metals in Dipterygophyllum dipterygophyllum did not exceed the standard limit value.The inoculation of inorganic phosphorolytic bacteria promoted the improvement of medicinal quality of Dianzhong Tower to a certain extent.According to the pharmacopoeia,the contents of the active components of saponin I,saponin II and saponin VII are 0.553mg/g～0.784 mg/g,1.131 mg/g～1.328mg/g and 0.477mg/g～ 0.640 mg/g.In comparison with the control group,the maximum increases were 41.77 %,8.52% and 42.54%respectively.Based on what has been discussed,inoculation of different inorganic phosphorus dissolving bacteria can promote the growth and development of Dipterocarpus dipterocarpus,reduce the use of phosphorus fertilizer,improve the rhizosphere soil microenvironment of Dipterocarpus dipterocarpus,enhance the quality of Dipterocarpus dipterocarpus as well as providing theoretical basis and data support for the economic and ecological benefits of artificial planting of Dipterocarpus dipterocarpus.The strain combination of Bacillus aryabhattai and Bacillus aryabhattai in the S6 treatment group was the most suitable for artificial cultivation of B.yunnanensis,so as to further study on field cultivation of B.yunnanensis.