Study On The Changes Of Myocardial Proteomics In Chronic Heart Failure With Deficiency Of Qi And Yin And The Intervention Mechanism Of Shenmai Injection Based On ITRAQ Technolog

Objective:(1)To study the changes of myocardial proteomics in rats with chronic heart failure syndrome of deficiency of xin Qi-yin,and to screen the differential proteins to explore the biological basis of this syndrome.(2)To study the changes of myocardial proteomics in rats with chronic heart failure treated by Shenmai injection and elucidate the mechanism of Shenmai injection in treating chronic heart failure from the perspective of protein.Methods:A total of 28 DAHL /SS salt-sensitive rats were randomly divided into normal group(8 rats)and model group(20 rats).Rats in the normal group were fed a low-salt diet,and rats in the model group were fed a high-salt diet(8% sodium chloride concentration)for 20 weeks.Four rats died in the model group,and the remaining 16 rats were divided into model group and Shenmai injection group according to random number table method,with8 rats in each group.The Shenmai injection group was given by intraperitoneal injection,and the model group was sterilized with water for injection.After 15 days of administration,myocardial samples of rats in the normal group,model group and Shenmai group were collected.Itopoisotope labeled relative and absolute quantification(i TRAQ)and liquid chromatography-mass spectrometry(LC-MS)were used to extract,qualitatively and quantitatively protein from the myocardial tissues of the three groups.Bioinformatics analysis was performed on the screened differential proteins.Results:1.Myocardial proteomics of rats with chronic heart failure syndrome of xin Qi-yinCompared with the normal group,there were 46 different proteins in the model group,among which 18 proteins were up-regulated and 28 proteins were down-regulated.Go analysis was performed on the differential proteins,and 150 biological processes were enriched,the top five of which were lipid metabolism,cellular or subcellular component movement,cell mobility,cellular lipid metabolism,and lipid catabolism.There were 24 cell localization of differential proteins,and the top five were extracellular region,extracellular region,extracellular space,endoplasmic reticulum,and membrane surface.There are 30 molecular functions of differential proteins,and the top five are signal receptor binding,sulfur compound binding,transport activity,amino compound binding,and polypeptide binding.2.Myocardial proteomics of rats with chronic heart failure syndrome of xinqi-yin treated by shenmai injectionCompared with the model group,there were 32 different proteins in the Shenmai group,among which 28 proteins were up-regulated and 4 proteins were down-regulated.By GO analysis of differential proteins,96 biological processes involving differential proteins were enriched.The top five were negative regulation of catalytic activity,negative regulation of hydrolase activity,positive regulation of endopeptidase activity,positive regulation of peptidase activity,and negative regulation of endopeptidase activity.There were 13 cell localization of differential proteins,and the top five were extracellular region,extracellular region,extracellular space,endoplasmic reticulum,and membrane surface.There were 12 molecular functions of differential proteins,and the top five were transition metal ion binding,endopeptidase modulator activity,peptidase modulator activity,peptidase activity acting on L-amino acids,and peptidase activity.Conclusions:(1)The myocardial protein profile of rats with deficiency of heart qi and Yin in chronic heart failure was changed.Differentiated protein indicated that the formation of this model was the result of the joint action of multiple biochemical pathways,which were mainly reflected in energy metabolism,protein synthesis and modification,fatty acid oxidation,REDOX,cell adhesion and cyoskeleton.SPG7,ACBD3,FABP3,HADH,NCAM1,ERO1 A played an important role in the formation of the model.(2)The mechanism of Shenmai injection intervention for heart failure mainly involves energy metabolism,kinin system and catalytic reaction.The expression callback of GSTM7,RPL24,ATP6V1E1 and LOC680329 after Shenmai injection intervention may become a potential therapeutic target.