Interventional Effect Of Ganwei Baihe Decoction On Pyroptosis Pathway Of NEK7/NLRP3 Cells In Rats With Stress Ulcer Mode

Objective:To explore the intervention effect of Ganwei B aihe Decoction on pyroptosis pathway of NEK7/NLRP3 cells in SU model rats by establishing SU rat model.Methods:40 SD male rats were randomly divided into normal group,model group,esomeprazole group(western medicine group)and Ganwei Baihe Decoction group(Chinese medicine group),10 rats in each group.After 3 days of adaptive feeding,on the 4th day of the experiment,the rats in the blank group and the model group were intragastrically administered with normal saline,the Esomeprazole group was intragastrically administered with Esomeprazole Enteric-coated Tablets,and the Ganwei B aihe Decoction group was intragastrically administered with Ganwei Baihe Decoction for 7 days.On the 10 th day of the experiment,all rats were fasted for 24 hours after the last gavage.On the 11 th day of the experiment,except for the blank group,the other three groups were modeled by water immersion-binding method.After modeling,the rats were sacrificed and the serum and gastric tissue were taken for examination.The gastric ulcer index and ulcer inhibition rate were calculated.The pathological changes of gastric mucosa were observed by HE staining.The contents of IL-1β and IL-18 in serum were detected by Elisa.The expression of NEK7,NLRP3 and caspase-1 protein was detected by WB and PCR.Results:1.Ulcer index:The model group was significantly higher than the blank group(P<0.0 1).The Western medicine group and the Chinese medicine group were lower than the model group(P<0.01).There was no statistically significant difference(P>0.05)between the Western medicine group and the Chinese medicine group.The ulcer inhibition rate in the Western medicine group was 41%,while the ulcer inhibition rate in the Chinese medicine group was 50%.2.HE staining:gastric mucosa inflammation of rats in the model group was obvious,while gastric mucosa inflammation of rats in the traditional Chinese medicine group and the western medicine group was reduced.3.Elisa test:Compared with the blank group,the model group’s serum content of IL-1β、IL-18 significantly increased(P<0.0 1).The Western medicine group and the traditional Chinese medicine group content of IL-1β、IL-18 was lower than that of the model group(P<0.0 1).The content of IL-1β bwtween the Traditional Chinese Medicine Group and the Western medicine group was no statistical difference(P>0.05),while the IL-18 content in the Chinese medicine group was lower than that in the Western medicine group(P<0.0 1).4.PCR:Compared with the blank group,the expression of NEK7,NLRP3,and caspase-1 in the model group was significantly increased(P<0.0 1).The expressions of NEK7,NLRP3,and caspase-1 in the Western medicine group and the Chinese medicine group were lower than those in the model group(P<0.0 1).The expression of NLRP3 and caspase-1 in the Chinese medicine group was lower than that in the Western medicine group(P<0.01),but the expression of NEK7 in the Chinese medicine group was lower than that in the Western medicine group(P<0.05).5.WB:Comparedwith the blank group,the protein expression of NEK7,NLRP3,and caspase-1 in the model group was significantly increased(P<0.0 1).The protein expressions of NEK7,NLRP3,and caspase-1 in the Western medicine group and the Chinese medicine group were lower than those in the model group(P<0.0 1).The protein expression of NEK7,NLRP3,and caspase-1 in the traditional Chinese medicine group was lower than that in the western medicine group(P<0.01).Conclusion:1.In SU model rats induced by water immersion and restraint,the expression of NEK7/NLRP3 pyroptosis pathway in gastric tissue is increased,while pyroptosis induces the level of serum IL-1β、IL-18 inflammatory factors is upregulated,the degree of gastric mucosal damage is increased,and erosive ulcers appear.2.Ganwei B aihe Decoction can alleviate gastric mucosal damage in SU rats,inhibit the occurrence of erosive ulcers,and reduce the expression of NEK7,NLRP3,caspase-1 proteins in gastric tissue,while downregulating serum IL-1β、IL-18 inflammatory factor levels.3.Ganwei B aihe Decoction can reduce the degree of gastric mucosal damage and ulcer in S U model rats by inhibiting NEK7/NLRP3 pyroptosis pathway.