Research On Electrochemical Raman Sensor For Homocystein

Homocysteine(Hcy)was important in the body’s metabolic system and was involved in the metabolic cycle of methionine and folate to maintain metabolic stability.However,elevated levels of Hcy in the body can lead to functional metabolic disorders,resulting in homocysteinemia and cardiovascular diseases such as Atherosclerosis(AS),which posed a serious threat to life and health.Hcy was one of the biomarkers of AS and abnormal levels can reveal the development and progression of AS.Therefore,rapid detection of Hcy is important in the early screening and mid-term treatment of AS.In this study,a biosensor based on electrochemical surface-enhanced Raman scattering(EC-SERS)technology was designed,which is used for the rapid detection of Hcy.It has the following main components.(1)A SERS sensor based on AgNPs@GFP was designed and prepared,which was used for the detection of melamine molecules.Glass fiber paper(GFP)was used as the substrate.Silver nanoparticles(AgNPs)were grown in situ on GFP by silver mirror reaction with the ascorbic acid reduction to form AgNPs@GFP films as SERS substrates.From the results,it was concluded that the size and density of AgNPs on GFP increased with increasing ascorbic acid treatment time.The factor gradually increased when SERS was enhanced up to a factor of 838.The sensor shows good linearity for melamine concentrations between 10 and 100 ng/m L.The fitted straight line was I=150*C+1096 with a detection limit as low as 1ng/m L.(2)An EC-SERS sensor with Au@Ag/GO/ITO as the EC-SERS substrate was designed and prepared,which was used for the detection of Hcy molecules.After ultrasonication was used to prepare the GO aqueous solution,the GO aqueous solution is spin-coated onto ITO to form GO/ITO thin film.Then,Ag/GO/ITO substrate was prepared on the GO/ITO film by self-assembly method.On this basis,electrodeposition was used to prepare Au@Ag/GO/ITO EC-SERS sensor.From the results,EC-SERS substrate prepared by electrodeposition for 30 min was found to have enhancement factors of up to and excellent charge transfer rates.In addition,differential pulse voltammetry(DPV)showed a good linear relationship with Hcy concentration in the range of 0.1-120 ng/m L,with the expression of I=1.73*C+24.26 and sensitivity of.The detection limit was as low as 0.06 ng/m L.At the same time,SERS spectroscopy revealed that the electrochemical oxidation product of Hcy was L-cysteine sulfinic acid,and the detection limit of Hcy using SERS was 0.2 ng/m L.In this study,the developed EC-SERS biosensor offers the advantages of low cost,high sensitivity,and rapid detection speed,facilitating the quick identification of Hcy molecules.This method will have broad applications in fields such as food safety and biomedicine...