| Hematopoietic growth factor and transcription factor are crucial for the differentiation and development of hematopoietic cells. In this study, M-CSF and EDAG are selected to explore the molecular mechanism of hematopoietic regulation.Macrophage colony stimulating factor (M-CSF) is a multiple functional cytokine not only for hemopoietic system but also for non-hemopoietic systems, such as breast function, pregnancy and osteoclastogenesis. Due to alternative splicing of M-CSF mRNA, M-CSF exists in at least three forms: secretary (s-M-CSF), membrane-bound (m-M-CSF) and extracellular matrix or porteoglycan (PG-M-CSF). All three forms bind to the same receptor, M-CSF-R. M-CSF-R is encoded by proto-oncogene c-fms, which is a ligand-inducible protein tyrosine kinase receptor.Some isoforms or mutations have been demonstrated including membrane bound and cellular M-CSF, which associated with atherosclerosis, chronic kidney failure, ovarian carcinoma and some leukemia, lymphoma and may be as a predictor factor for tumor or other diseases.J6-1 is a leukemic cell line with the character of Hodgkin's disease (HD) cell line, which appears heterogeneity. This cell line exhibits density-dependent and cluster-forming growth characteristics. We previously reported that the membrane associated factor (MAF-J6-1) and its receptor was found from human leukemic cell line J6-1. Our study suggested that MAF-J6-1 was an M-CSF like factor, but its gene hasn't been cloned.In this study, two fragments were amplified by RT-PCR from J6-1 cells using M-CSF specific primers. One is 791 bp, which designated as Ml, and the other is 1685 bp, which designated as M2. Using c-fms cDNA specific primers, a 2932 bp fragment (which designated as MR) was amplified by RT-PCR from J6-1 cells. The sequencing results showed that Ml has a 768 bp ORF, showing 99.2 % homology...
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