Analysis Of Ecological Structure And Function On Integrated White Rot Fungi Technology For Treatment The Actual Dyestuff Wastewater

White rot fungi is capable of oxidizing persistent organic pollutants (POPs) including dyes because of their strong biodegradability and unique metabolic type. However, It's hard to promote the comprehensive ability of treating actual dye wastewater, which will affect its applications and effectiveness. In addition, there are many factors influencing the growth and ligninases production of white rot fungi, as well as the enzymatic reaction, which raise new challenge to the technical process of white rot fungi applied to treating actual dye wastewater.This thesis is based on the project of effective microorganism techniques in wastewater treatment supported by Chinese National Programs for High Technology Research and Development (863Program). In the current study, the ligninases production of four white rot fungi was studied. The six carriers for immobilization of P. C. were also researched. Then the small-scale of integrated white rot fungi reactor was constructed and the performance of this reactor was researched with different conditions. And finally, the pilot-scale field tests were carried by Shanghai No.8 Dyestuff Chemistry Plant. The purpose of this thesis is to provide certain scientific references for the construction of white rot fungi integrated reactor and its stable and effective running.Four white rot fungi, G. Sinense, C. versicolor, G. lucidum HG and P.C., were significant different in enzyme activity of MnP and LiP. Both MnP and LiP of P.C. had the highest enzyme activity, for the peak activities of MnP and Lip were 225.67 U/L (detected at 5th day) and 110.35 U/L (detected at 6th day) respectively. The enzyme activities of P. C. under N-limited culture condition were much higer than under C-limited culture condition. Statistical analysis using spss18.0 indicated that the MnP and LiP enzyme activities of P. C. under Nitrogen and Carbon limited culture conditions exhibited significant differences, which p value less than 0.05. The enzyme activities of MnP and LiP of P.C. were comparatively high under pH value 4.0 to 4.5, and the two enzyme activities reached peak at pH value 4.5. MnP (more than 240 U/L) and LiP (more than 110 U/L) were at the peak activites when medium volume was 250mL. MnP and LiP reached the peak of 180 U/L and 130 U/L respectively when inoculation amount of spore suspension was 1.3×106 cell/mL. Speed between 120r/min and 140r/min was favorable for higher activities of MnP and LiP. Consequently, with respect to the four white rot fungi, MnP and LiP produced by P.C. had the highest enzyme activities.P.C. immobilized on bagasse and corn cob which was two agricultral wastes exhibited higher activities of MnP and LiP than on other four immobilized carriers. Through analysis by spss18.0, the activities of MnP and LiP produced on bagasse and corn cob were dramatically different from the situations of others, with corresponding p=0.000<0.01. The activites of LiP on bagasse were all higher than those on corn cob, despite the activities of MnP on bagasse were slightly less than those on corn cob. Taken these factors into consideration, bagasse was employed as immobilized carrier for the following lab-scale and pilot-scale experiments. When the immobilized carrier bagasse was 6 g/L, the maximum MnP could be 157.1U/L. Meanwhile, its decoloration was optimal, reaching 99.6%at the end of the experiment. Accroding to spss analysis, the dosage of bagasse and decoloration rate were extremely significant correlated, with correlation coefficient r is 0.373, p value less than 0.01. In addition, other bacterials were inhibited effectively under the above bagasse dose. SEM observation indicated that bagasse could be used well as carriers for P. C. adherence and growth.Using the orthogonal optimization test to determine the three important parameters (T, DO and pH) of intergrated white rot fungi reactor. The variance analysis showed that T has the grwatest impact on reactor performance, followed by DO, pH. When T, DO and pH was respectively 30℃,5 mg/L and 5, the system obtained the highest decolorization efficiency. However, when integrated white rot fungi treating the dyestuff wastewater, the colority and CODCrwas reduced rapidly within 24 h. According to the analysis, the HRT was determined 24 h.The removal efficiency of CODCr and BOD5 was the best when the ratio of dyestuff wastewater and the N-limited liquid medium was 4:1. The average removal of NH3-N can reach the national top standard, lower than 15 mg/L. The colority of the actual dyestuff wastewater was also reach the national standard lower than 50. Meanwhile, the average concentration of HgCl. was 0.058 mg/L, belonging to low toxicity level. The corresponding fungal microbial community diversity index stabilized around 5.60 and the P.C. is the dominant species all the time. Whether diluting or not, the reactor can't effectively improve the removal efficiency of CODCr, BOD5 and reduce the biotoxicity of the actual dyestuff wastewater. Microbial community diversity index of the reactor was stable at around 5.48. Under the condition of low temperature, the removal efficiency of CODcr, BOD5, biotoxicity and decolorization rate was lower than normal. Microbial community diversity index of the reactor was lower than normal temperature about 5.20. Meanwhile, with the system running, microbial community diversity index was increasing in the latter of the condition of low temperature. In accordance with DGGE profiles, it indicated that other bacteria intruded the system in the latter run, but not the dominant species.The pilot study indicated that the intergrated system have good removal efficiency of NH3-N, CODCr and BOD5. Meanwhile, the intergrated system also had good removal efficiency on the actual toxicity of wastewater. The average concentration of HgCl2 in the effluent had been reduced to 0.08 mg/L, of low toxicity level.