Research On Extreme Thermophilic (70℃) Two-Phase Anaerobic And Combining Process For Treating High Concentration Organic Wastewater

Up to now, the anaerobic treatment process is still play an important role in the high concentration with organic wastwater from food industrial. But there is not a practically feasible and reliable technique for the high temperature, high concentration, complicated wastewater containing protein from corn processing enterprise.The hard-degradation organic pollutant and the high-concentration sulfate are the most difficult problems in the wastewater. 1 Research purpose and content The high temperature, high concentration, complicated fractions starch wastewater from corn processing enterprise was treated as a subject in researching extreme temperature sludge domestication and culture technique as well as combining technology process, which was made up of innovative extreme temperature 70℃hydrolyze acidification technology, immobilization acidification phase technology, extreme temperature two-phase anaerobic technology , high temperature aerobic technology and biological activity carbon(BAC).The thesis has revealed the physiological characteristic of the dominant bacterium lived in extreme temperature, change characteristic and mechanism of the factors that influence the acidification in extreme temperature, explained the treatment effect of the two-phase anaerobic reactor in extreme temperature, established the evaluating indicator system of effect on the extreme temperature acidification, and exploited an innovational treatment technology. The research was divided into three parts: the first part attached importance to study on the effect and mechanism of two-phase extreme temperature anaerobic treatment from the point of view of method, research on high temperature treatment and reusing technique of the wastewater from the point of view of the wastewater and primary discussed the assessment index system and evaluation method for hydrolysis acidification of the anaerobic treatment; the second part attached importance to study on the effect and mechanism of immobilization acidification and the mechanism of two-phase extreme temperature anaerobic treat. 2 The mind results 2.1 Results obtained from the study and evaluation on effect of acidification at extreme temperature (70 ) for treating Corn-Starch wastewater The effect of upflow acidification reactor, seeded with mesophilic granular sludge, at extrem temperature(70 )treating corn –starch wastewater was the focus of a comparison study ,the performance of similarly-fed of 35 and 55 reactor was also tested. At three conditions ,the characterictics of COD,SS, alkalinity,pH VFA, SO2-4 ,S2-and proteion changes in acidification process and infuence factors were studied The result showed that 70 reactor achieved 28.5% acidification radio VFA as COD/g of influent COD and 0.142 h-1 acidification rate(Kh) and the COD removal was about 24.% ,SS removal :86% and Proteion hydrolysis ratio was 89.4% at a high VLR of 36.2 Kg CODm-3.d-1.in continous flow test and SO2-4 removal was 32.4 %.The paper also proposed to assessment the acidication reactor performance using acidification ratio and acidification rate and proven that it was a effictive and feasility as an comprehensive evaluation index system. 2.2 Results obtained from the two-phase of acidification and UASB at extreme temperture (70 ) for treating wastewater containing protein The effect of upflow acidification reactor(UAR) and anaerobic reacotor,(UASB) , seeded with mesophilic granular sludge at extrem temperature(70 )treating wastewater containing protein was the focus of a study at different COD , different VLR and different C/N..of influent . At the conditions ,the characterictics of COD, VFA and VFA/COD,-and protein changes in acidification process and UASB were studied . The result showed that 70 acidification reactor achieved 52.7% acidification radio VFA as COD/g of influent COD, 95% proteion hydrolysis ratio, 47%COD removal rate(%) in UAR and 95% in UASB .Total COD removal rate in two phase was 97% at low load. 57% COD removal rate in UAR phase and, 88.1% in UASB phase at high load with VLR of 35 Kg COD/m-3.d. had been achieved. When C/N was changed from 14/1 to 300/1, COD removal rate in UAR phase was decreased obviously while it was increased in UASB . The total COD removal rate in two-phase was above 90%. The paper also assessment the performance of UAR and UASB using acidification ratio and COD removal rate, and proven that it was effective and feasility as a method treating wastewater containing protein.2.3 Results obtained from the Extreme Thermophilic(70 ),Combining Two-Phase Anaerobic/Activated sludge/Bio-Activated Charcoal for Treating Food Wastewater This study was conducted to investigate the performance of combining two-phase anaerobic(TPA)/activated sludge(AS)/bio-activated charcoal(BAC), seeded with mesophilic sludge,for treating food wastewater at extreme temperature(70 ) to prove an useful and new treatment process to allow treated water to be reused for closed heat water circuits.The system had a total hydrulic retention time of 42h. Reactor performance was characterized by COD, VFA, pH, amino-acide. An oreall COD treatment efficiency of 99.62%, VFA and amino-acide of 100% and pH between 6.6-7.5. were observed for the system.COD removal of acidification phase, methane phase, activated sludge and BAC was repectively 47.7%, 33.7%, 14.5%,and 1.1%.VFA removal of methane phase,activated sludge and BAC was respectively 56%, 21.2% and 21.8%. Amino-acid removal of methane phase, activated sludge and BAC was respectively 34.8%, 62% and 3%.COD concentration of effluent in the system was lower than 50mg/L.An additional experiment shown that activated sludge and bioactivated charcoal at 70 may allow COD concentration of influent up to 3500mg/L and COD removal was up to 95.8% at high organic loading 4.5kgCOD/m3.d with aerobic conditon. 2.4 Results obtained from the Hydrolysis of Wastewater Contained Protein by Immobilized Protein Enzyme Protein-enzyme had been immobilized on big-hole resin ,activated charcoal and silica particles by a absorption method. This study focused on effect of enzyme immobilization, thermal stability and hydrolysis efficiency of wastewater. The result of batch test shown that the ratio of immobilized enzyme on activated charcoal, big-hole resin and silica particles was 61.95%, 37.85% and 20.31% respectively. The highest of thermal ability of activated charcoal, big-hole resin and silica particles was 18.18mg/min, 21.48mg/min and 3.6mg/min respectively at pH 5.5.The total amino-acids of big-hole resin and activated charcoal immobilized enzyme after hydrolysis was respectively increased to 21.26 times and 14.6 times, The kind of amino-acid was increased from 14 to 16 kinds. The continuous-flow test result indicated that concentration of substrate in influent had significant effect on the hydrolysis rate of immobilized enzyme reactor, The reactor was able to gain hydrolysis rate of 80% for protein and 36.1%(VFA/COD%) for organic materials in the wastewater with a moderate concentration of protein (about 500mg/L). The ethanol and acetic of volatile hydrolysis-mixture composition were 50.3% and 33.3% respectively. Thebig-hole resin was a optimal carrier of using the immobilized protein enzyme of to hydrolyze protein particles in wastewater.as stated. above. 2.5 Results obtained from the Two-phase of immobilization enzyme acidfication and UASB reactor for wastewater treatment This paper presented exploratory results on the association of acidfication reactor, which filled by the big-hole resin immobilized protein enzyme, and UASB seeded with mesophilic granular(70 ) as a methane phase for corn starch wastewater contained protein. Continus experiments were conducted to assess the effect of acidfication for VFA production and COD removal in different COD, load and C/N of influent. The results shows that acidfication rate(VFA/COD) was consistently over 30% in acidfication-phase at a moderate C/N, protein hydrolysis rate was up to 95% and pH of enfluent was between 6.7 and 7.0; COD removal was over 91.3% at low C/N. The C/N of influent had significant effect on hydrolysis of acidfication phase and COD removal of methane phase. 2.6 Results obtained from the Organic Acids Conversion in Two-phase of Immobilization Enzyme Acidfication and UASB, Anaerobic Reactor for Wastewater Treatment Using immobilization enzyme acidification phase and UASB productuin methan phase as two-phase anaerobic fermentation process, the change characteristics and the change rate of organic acid and ethanol organic, composition and fermentation type as well as COD removal were studied. The experimental results showed that acidfication rate(VFA/COD) was consistently over 28.2% . The concentration of ethanol , acetic, butyric and propionic in acidfication-phase were 44.8 , 38.4 , 9.8% and 6.9%,respectively, as follow: ethanol> acetic>butyric>propionic. The removal of ethanol, acetic, butyric and propionic in methane production phase were 99.8%,92.0%,59.9% and 46.0%, respectively, and the removal of COD was higher than 90%. The conversion velocity of substrate in the methan production phase showed as follow: ethanol >acetic> butyric > propionic. The fermentation type of the acidification phase was ethanol-type fermentation. The effect of each acid and ehanol conversion on COD removal was as follow: ethanol>acetic> butyric > propionic. 2.7 Results obtained from Studies on isolation identification and morphology characteristics of anaerobic strain from extreme thermophlic (70 ) anaerobic reactor Some bacterium were isolated from anaerobic sludge of acidification and methan phase at 55 and 70 . According the anaerobic development, stain and micrograph check , the results showed that there were differences between 55 and 70 with acidification and...