Studies On The Instability Of Anthocyanin Accumulation In Suspension Cultures Of Vitis Vinifera

The instability of secondary metabolite production in plant cell culture is one of the bottlenecks of its commercialization. In order to understand this instability, a systematic investigation of anthocyanin accumulation in suspension cultures of Vitis vinifera, as a model system, had been initiated in our laboratory. Phenomena of instability of anthocyanin accumulation and the factors that affected the instability had been studied. And based on the development of a homogeneous cell line, the relatively high and stable production of anthocyanin had been realized in suspension cultures of V. vinifera.By investigating the biomass and anthocyanin accumulation in suspension cultures of V. vinifera, the maintenance medium, the culture conditions and the process that could significantly increase anthocyanin yield had been optimized. There had been 4 kinds of instable phenomena in anthocyanin accumulation in suspension cultures of V. vinifera. Firstly, gradual losses in anthocyanin contents were observed in long-term subcultures. The variation coefficients were 54-84 %. Secondly, the fluctuations of anthocyanin accumulation had irregular cyclic patterns. Thirdly, the anthocyanin biosynthesis was instable in repeated flasks within the same subculture. The variation coefficients were 4-17 %. Fourthly, the anthocyanin components behaved instable. The heterogeneity of the cultures was the root of the instability.Inoculum size had a more remarkable effect on instability of anthocyanin...