Toxicity Of Avermectins To Rosy Barb (Puntius Conchonius) And The Gill Cell Line Of Flounder (Paralichthys Olivaceus)

Of all the environments, the aquatic ecosystem is at greatest risk from pollutants since all chemicals, including pesticide, whether initially released on land, into the atmosphere or directly into rivers will eventually find themselves in the rivers and oceans as the final repository. After these pollutants enter into the water body, they will exert destruct effect on the non-target organisms in the aquatic ecosystem especially fishes, in turn, enter the entire food chain, and ultimately severely affect the health of human beings. Hence, aquatic environment pollution and toxicity to fish or other aquatic organisms by insecticide have always being the concern in the world. Rosy barb, a member of the Cyprinidae family, and FG-9307 cell line from the gill of flounder were used to examine the toxicity of avermectins by in vitro and in vivo toxicity assay in this study.The first part examined the effect of abamectin on the motility and ultrastructure of rosy barb sperm. The main findings are (1) Abamectin exerts deleterious effects on rosy barb sperm. abamectin is capable of impairing the sperm motility at 0.14μmol/L. (2) The toxicity of abamectin to rosy barb sperm is both dose dependent and exposure time dependent. As the concentration of abamectin increased and the time contacting with abamectin prolonged, the sperm motility of rosy barb declined significantly. (3) The ultrastructural examination showed that most of the sperm exposed to 0.14μmol/L abamectin for 30 min remained morphologically normal, while the midpiece and its surrounding mitochondria of the sperm treated with 0.28μmol/L abamectin for 30 min were severely damaged. For the sperm treated with 0.42μmol/L abamectin for 30 min, the majority of the sperm were abnormal. Sperm heads were dissolved and the...