Toxic Effects Of Fenpyroximate On Flounder Paralichthy Olivaceus And The Gill Cell Line Of Flounder

Fenpyroximate is a kind of mitochondrial electron transport inhibitors and acaricide, which have been developed by Japan from 1990s', and now has been used more and more widely all over the world because of its high selective acaricide effect. Studies on the toxic metabolism and remaining of fenpyroximate in the environment show that, fenpyroximate could remain in soil and water for a relatively long period, and all the pesticides which have the same toxic mechanism to fenpyroximate show high toxicity to fish, and are closely related to human Parkinson's Disease. So more and more attention has been paid to the toxicity of fenpyroximate on water environment, aqueous organisms, and human health. In this study, Flounder Paralichthy olivaceus and its gill cell line FG -9307 were selected as in vitro and in vivo testing systems, acute toxicity of fenpyroximate to this economical marine fish species was investigated, and the correlation of toxicities between these two testing systems were also discussed .In vitro toxicity of fenpyroximate to FG cells was investigated at first. The results showed that: (1) 48h IC50 tested by three methods was 0.48-0.51μmol/L, and 96h IC50 was 0.89-1.25μmol/L, indicating that fenpyroximate was toxic to in vitro fish cells, or FGl cells were highly sensitive to fenpyroximate; (2) fenpyroximate showed obvious inhibition effect on the proliferation of FG cells in the concentration range from 0.1 to 30μmol/L, and can cause FG cells obvious morphological changes; (3) fenpyroximate affected the activities of antioxidant enzyme system and the concentration of superoxide anion in FG cells. FG cells exposed to fenpyroximate exhibited prominent inhibition on the enzyme activities of SOD, CAT and GPX, and obvious increase of the concentration of superoxide anion in FG cells.Second, the in vivo toxicity of fenpyroximate to flounder was investigated by assay of the antioxidant enzyme activities and histochemistry examination. The results showed that, (1) 48 h and 96 h LC50 of fenpyroximate to young flounder were 28.77 nmol/ L and 11.74 nmol/ L, respectively; (2) fenpyroximate influenced r the antioxidation enzyme activities of flounder liver. the activities of SOD, CAT and GPX in the liver of flounder exposed to fenpyroximate increased obviously at the first several exposure hours and then were inhibited to be a level lower than that of control; (3) fenpyroximate can cause obvious damages to the structures of gill and liver of exposed flounder.In the end, we also analyzed the results of the toxicities of environmental pollutants using FG cell line, rosy barb(Puntius conchonius) and flounder as in vitro and in vivo toxicity testing systems, which have been carried out in our lab and investigated the correlation between in vitro and in vivo bioassay systems. The results indicated that the in vivo toxic testing system is more sensitive than in vitro system, and more data are needed to make a conclusion on the correlation of in vitro (FG cells) and in vivo (flounder) toxicity testing system...