Study On The Novel Optical Detector For Capillary Electrophoreses And Its Application

High performance capillary electrophoresis (HPCE) has received a great amount of attention as an attractive separation technique in recent years due to the following characteristics: simplicity, high efficiency, low cost and short analysis time. At present, HPCE has been applied in diverse fields, such as life sciences, biomedicine and environment detection. However, the exceptionally small solute section in the capillary electrophoresis resulted in the short light path, CE separations coupled with highly sensitive detector had become more and more necessary. So, the research of detector was very important for developing the CE methodology. To date, some employed CE detectors were following: ultraviolet visible, laser induced fluorescence, chemiluminescence, mass spectrum, nuclear magnetic resonance and electrochemistry detector. The laser induced fluorescence and chemistry luminescence were the most sensitive detector among the above detectors. But, the conventional laser induced fluorescence detector must make use of laser as the excitation source, such as argon ion and helium-cadmium laser. These lasers suffered from limit of alternative excitation wavelength, expensive cost, bulky volume, short lifetime and high power consumption, which limited the application of capillary electrophoresis laser induced fluorescence detection methodology. For the capillary electrophoresis chemiluminescence detection, many detectors had been developed. However, the highly sensitivity detection was focused on the inorganic...