| As the fast development of economy, animal derived food is becoming more and more important in our life, but hormone residues in every kind of animal derived food has become a hot topic of public health. In this paper, a comprehensive analytical method based on ultra performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-MS/MS) with negative mode has been developed for the simultaneous determination of glucocorticoids residues in animal derived food and urine. Multi-reaction monitoring mode was employed for the determination. Samples were extracted with methanol after the buffer solution was added, then defatted with n-hexane (except urine samples). Sample concentration and purification were performed using GCB and amino-propyl solid extraction cartridge. The separation was performed on a Waters ACQUITY UPLCTM BEH C18 column with a gradient system of methanol and water (containing 0.1% formic acid) as mobile phase at a flow rate of 0.3 ml/min. The qualification analysis was done by using retention time and distribution of diagnostic ion pairs, and the quantification analysis was based on the peak intensity of parent ion and a fragment ion with higher signal. The detection limits (LOD) of the method were from 0.003 to 0.38μg/kg and the limits of quantification (LOQ) ranged from 0.011 to 1.27μg/kg, average recoveries of glucocorticoids (spiked at the levels of 2, 0.4μg/kg) ranged from 81.0% to 112.3%, with relative standard deviations between 1.8% and 16.6%. Routine tests showed the method was fast, sensitive and specific for the glucocorticoids residues determination.The separation for betamethasone and dexamethasone was performed on a Waters ACQUITY UPLCTM BEH C18 column with a gradient system of acetonitrile and water (containing 0.1% formic acid) as mobile phase, and an analytical method based on UPLC-MS/MS has been developed for the simultaneous determination of betamethasone and dexamethasone residues in animal derived food and urine. The...
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