Osteogenic Ability Of Autogenous Cells With Gene Transfection And Porous Calcium Phosphate As A Scaffold

Osteogenic Ability of Autogenous Cells with Gene Transfection and Porous Calcium Phosphate as a Scaffold Tissue engineering is an interdisciplinary science of co-development and combination of modern cell biology, biomaterial science and engineering. The establishment of tissue engineering technique is a breakthrough over conventional organ transplantation to restore and reconstruct the function and structure of tissues lost to the injury and disease. In the light of present knowledge, the formation and repair of bone with tissue engineering repuires study on: 產 source of pluripotential mesenchymal stem cells capable of differentiating into osteoblasts; ゞrowth and differentiation factors that direct these cells to migrate into the osseous defect, to proliferate, and to differentiate into osteoblasts; ゛ bioresorbable matrix or scafold to support the attachment and migration of these cells into the osseous defect; and ゛ngiogenesis and the formation of a vascular network throughout the newly formed bone. The present study is based on these requirements and contains four main parts: 1 To observe the osteoblastic proliferation, differentiation and osteogenetic phenotype of rat bone marrow stromal cells in a a -MEM supplemented with L- ascorbic acid, Na P -glycerophosphate and Dex. It seems that synthesis of mineralized bone-like nodules and collagen in vitro requires the presence of ascorbic acid and P -glycerophosphate in the culture medium. The presence of Dex benefits to cell differentiation but suppresses its proliferation. To obtain endothelial cells from rat bone marrow with discontinuous gradient centrifugation on Percoll. The characteristics of the rat bone marrow endothelial cells in vitro are consistent with those of vascular endothelial cells with the presence of Weibel-Palade bodies. 2 A recombinant human morphogenetic protein 2 (BMP-,) plasmid was duplicated, purificated and transfected into bone marrow stromal cells (MSCs) of rat. It appeared that rhBMP2 mRNA was successfully transcripted and BMP2 expressed in transfected cells from the positive results of in situ hybridization, ?? immunohistochemistrical staining and Western blot. 3 To investigate the effects of fibronectin surface modification on osteoblasts adhesion to porous calcium phosphate bioceramics. it seems that the fibronectin surface coating is beneficial to osteoblast anchorage and its osteogenic phenotype. lb establish a Sprague-Dawley rat model of bone tissue engineering for investigating the ability of heterotopic osteogenesis and critical size calvarial defect repair with autogenous osteoblasts loading on porous calcium phosphate ceramic. It seems that the experimental results got from this closed colony animal model were much close to clinical situation superior to those from other homogenic or immune deficiency animals. To observe heterotopic osteogenesis of autogenous marrow stromal cells with rhBMP2 gene transfection and loading on porous calcium phosphate ceramic scaffolds in a Sprague-Dawley rat model. It seems that autogenous stromal cells with rhBMP2 transfection act as a bioreactor promoting proliferation and differentiation of stem cells when they are replanted into the corresponding animals. Gene engineering and tissue engineering technique was utilized to compose a cell-ceramic hybrid material for repair of a Sprague-Dawley rat model of critical size calvarial defects. It...