| Acute pancreatitis especial severe acute pancreatitis (SAP) remains one of the most challenging common diseases which affllicts man.That such a small organ as the pancreas can be affected by an acute inflammatory process which has far reaching consequences for multiple organ s throughout the body and which fascinated doctors of many disciplines over the past 150 years, still remains remarkable.Despite important advances in understanding the causes of AP, the approach to prognosis and treatment have evolved largely through empirical processes rather than neat logical developments between pathophsiological processes and clinical practice.According to the the remarkable advance recently made in the earliest pathophysiological events such as acinar cells, the pancreas itself and the distant organs ,we have made the basic and clinical research of the management of SAP.The present study consists two parts:PartiThe study of the effect of rat NF- K B P65 antisence in experimental severe acute pancreatitis in ratsBackground and Aim:Death in the early stages of severe acute pancreatitis (SAP) is frequently the results of multiple organ dysfunction sysdrome (MODS) by systermic inflamatory response syndrome (SIRS) ,but its mechanism isnot clear. The aim of this paper is to investigate the state of nuclear factor -KB (NF- K B) in the tissue of pancreas. lung and kidney with SAP, and to assess the effectiveness of rat NF- K B P65 antisense on the pathology of pancreas > the serum cytokines. NF-K B P65 and phospholipase A2 (PLA2) expression in pancreatic, lung and renal tissue .Methods:(D SAP model was produced by the method of Aho et al.Under anaesthesia ,a laparotomy was performed and 5% sodium taurocholate (1 ml/kg body weight) was injected into biliopancreatic duct at a rate of 0.2 ml/min by microinfusion pump. As for severity of SAP , the pancreatic tissue was removed after death under anaesthesia , the tissue was fixed in 4% paraffin embedding and preparation of paraffin sections (4 u m) ,sections of each pancreas were stained by hematoxylin/eosin (H.E.). The severity of pancreas were determined by Spormann meathod. (2) Rat NF- K B P65 antisense (number of bases : 21 ,daltons:6456, 5"> GGGAAACAGATCGTCCCATGGCO') was synthesized by manmade by BioAsia (Shanghai China ) . (3) 90 SD rats were divided into five groups randomly : group 1 ( control group ), 10 SD rats who received false operation and normal saline (NS) via intraperitoneal (I.P.);group 2 (SAP group), 20 SD rats who received NS via I.P. after induction of the SAP model ; group 3 (NF~ K B group ) ,20 SD rats who received Rat NF-K B P65 via I.P. one hour before the induction of the SAP model ; group 4 (NF- K B P65 antisense pretreated group ), 20 SD rats who received Rat NF- K B P65 antisense (contration:0.5mg/ml,l ml/kg body weight) via I.P. one hour before the induction of the SAP model ;group 5 (NF- K B P65 antisense treated group) 20 SD rats who received rat NF- K B P65 antisense (contration:0.5mg/ml,lml/kg body weight ) via I.P. after the induction of the SAP model . ?Effects of rat NF- K B P65 antisense on NF- K B P65 and pancreatic PLA2 expression in the tissue of pancreas,lung and kidney of the rats were detected by immunohistochemistry methods. For immunohistochemistry , sections of each pancreas . lung and kidneywere evaluated on coded sections by monoclonal rat anti NF- K B P65 and rat anti PLA2 . The first antibody were diluted 1:100. (D Effects of rat NF- K B P65 antisense on the serum cytokines and serum pancreatic PLA2 /AMS of the rats were studied.TNF- a , IL-1 (3 , IL-6, IL-10 and IL-2 were assessed by enzyme-linked immunoassay (ELISA).The serum amylase and phospholipase A2 were measured .Results:?As for control group (group 1) was normal by nakedeye and microscope. Severity of pancreatitis in the other groups was messured at the point of 12,24 hours : the score of pancreatic pathology by Spormann in Group 2, 3 , 4 and 5 was (23.6 ?.6, 21.4?.8)> (20.7 + 3.4, 22.1 + 1.5), (8.7+1.8,...
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