Research On The Anti-epilepsy Constituents Of Stellera Chamaejasme L. And Citrus Grandis Osbeck

Upon the basis of experience on folk medicine, two kinds of plants, Euphorbia fischeriana Steud and Stellera chamaejasme L., used as "langdu" were confirmed to have the anti-epilepsy activity through pharmacodynamical study. S. chamaejasme L. showed better effect than E. fischeriana Steud. Major chemical constituents of the active site of S. chamaejasme L. were flavonoids and coumarins. According to the structural type and literatures, we think that the peels of Citrus grandis Osbeck may have similar effect. Pharmacodynamical results confirmed our hypothesis.24 compounds and 16 compounds were isolated from the active sites of the roots extract of Stellera chamaejasme L. and the peels extract of Citrus grandis Osbeck, respectively. Compounds from Stellera chamaejasme L. are neostellerin (1), isomohsenone (2) , stelleranol (3), 7-methoxyneochamaejasmin A (4) , mesoneochamaejasmin A (5), (+)chamaejasmine (6), 1, 5-diphenyl-1-pentanone (7), 1, 5-diphenyl-2-penten-l-one (8), p-coumaric acid w-eicosanyl ester (9), caffeic acid n-dueicosanyl ester (10), wikstrol A (11), wikstrol B (12), (+) -afzelechin (13), bursehernin (14), chamaechromone (15), mohsenone (16), neochamaejasmin A (17), neochamaejasmin B (18), 7-methoxychamaejasmine (19), chamaejasmenin B (20), daphnoretin (21), umbelliferone (22), scopoletin (23), S- ( + ) -1, 5-diphenyl -3-hydroxy-1-pentanone (24). Compounds from the peels of Citrus grandis were aurepten (25), isoimperatorin (26), marmin acetonide (27), pranferin (28), isomerancin (29), xanthotoxol (30), umbelliferone (22), meranzin hydrate (31), 8-(3-β-D-glucosyl-2-hydroxy-3-methyl- butyl) -7- methoxycoumarin (32), scopoletin (23), roseoside (33), friedelin (34), 5-hydroxmethylfuraldehyde (35), linonin (36), nomilin (37) and narigin (38). Among them, compounds 1, 2, 3, 4, 5, 6 were determined to be new compounds. Compounds 7, 8, 9 and 10 were new natural products. Compounds 11, 12, 13, 14 were first isolated from Stellera genus. Compounds 25, 26, 27, 28, 30, 33, 34, 35 were first isolated from Citrus grandis Osbeck. Among the above compounds, 22, 23 are the common constituents of the two plants.The existence of isomohsenone (2) and mohsenone (16) in Stellera chamaejasme L. proved Niwa's hypothesis that chamaechromonoids were transformed from chamaejasminoids.The research on chemical constituents and pharmacology were concluded.On the basis of our research on Stellera chamaejasme L. and the literatures, the spectral and structural characteristics, the stereochemical researching methods, the distribution in the 3 subtribes of Thymeleaceae and chemotaxonomy of biflavonoids were concluded. We found that biflavonoids played a very important role in the chemotaxonomy of Thymelaeaceous plants. We got a conclusion that only biflavonoids bearing flavan moiety existed in Daphne gemus, that biflavanones and chamaechromonoids existed in Stellera genus, and that biflavanones and biflavonoids bearing flavan moiety existed Wikstroemia species. The opinion that biflavanones only existed in Stellera genus was corrected and they were also found hi Wikstroemia genus. According to the distribution of biflavonoids, we support the viewpoint of classifying the Thymelaeaceae family into three subtribes: Wikstroemiinae, Daphninae and Passerininae.The anti-convulsive experiments suggested that the biflavonoids didn't show any anticonvulsive activity, but that umbelliferone, which is the common constituent of these two plants, could delay the convulsive stroke.