| Background: Laminectomy is one of frequently-used operation mode in spinal surgery, Coloboma caused by laminotomy is restored by fibrous connective tissue hyperplasia through inflammation reaction, granulation tissue production and scar tissue production basic pathology course. Foundation is a series of activity of inflammation cell and restore cell. Scar tissue hyperplasia and adhesion are importance cause of failed back surgery syndrome (FBSS) and influence curative effect of spinal operation seriously. To search and develop new material and method to prevent scar tissue hyperplasia and adhesion in canales vertebralis is a problem need to be resolved urgently in spinal surgery, native and foreign scholars have done a lot of useful research in basal experiment and clinic practice, and have made some progress. But the material and method which have definite effect and been widely used in clinic are not more. Spinal injury patients often need medication after operation. Long time local intramuscular injection not only increase pain to patient, but also decrease the medicine absorption, affect the curative effect. There has not been a material which could prevent peridural fibrosis and delay the release of medicine.Objective: to develop a new self degradation compound biomembrane of peridural fibrosis prevention and slow release drug.Method: The study be divided into the following four aspects1 -. DL-PLA/CHI biomembrane degradation study in vitroMethod of practice: Quantitative DL-PLA powder dissolved in acetone, quantitative CHI powder added to, to obtain three different proportional DL-PLA/ CHI compound (DL-PLA:CH3 ratio respectively is (9: 1) (8: 2) (7: 3)) , intensive mixing, pour into flat bottom container. After acetone volatilization , to be made into flap thick 0.5mm. at last to be cut into 40 mm X 20mm X 0.5 mm for experiment. By-5-same method, to made DL-PLA for experiment. Put flap immerse into 30ml physiological saline, place in 37癈 thermostat, soak 2Ws-16Wst to measure PH of degradation liquid; the change of aspect and shape; the weight loss; DL-PLA molecular.Result: 1) PH: PH of DL-PLA degradation liquid has no notable change in previous 12 weeks. PH value reduces rapidly after 12 week, reduces to 2.68 at 16 week. PH of DL-PLA / CHI degradation liquid has little increase in previous 12 weeks, the higher content of CHI, the higher PH value. PH value reduces also rapidly after 12 week, reduces to 2.74~3.24 at 16 week. 2) weight loss: weight loss of DL-PLA is the least along with time. The more CHI, the more weight loss of DL-PLA/CHI along with time. 3 ) molecular weight: Mv of DL- PLA in DL-PLA/CHI is higher than Mv of pureDL- PLA, the more Cffl, the higher Mv of DL- PLA in DL-PLA/CHI. This show the DL- PLA degradation velocity in DL-PLA/CHI than pure DL-PLA. Half-life period of pure DL- PLA is 4 weeks, half-life period of DL- PLA in DL-PLA/CHI is 4 ~8 weeks. CHI has inhibitory action to DL- PLA degradation.2^ Slow release study of biomembrane as drug carrierMethod of practice: Quantitative DL-PLA powder dissolved in acetone, quantitative CHI powder added to, quantitative Methylcobalamin added to at avoiding light condition, to obtain three different proportional DL-PLA/ CHI / Methylcobalamin and DL-PLA/Methylcobalamin compound, intensive mixing, pour into flat bottom container. After acetone volatilization , to be made into flap thick 0.5mm. at last to be cut into20 mm X 20mm X 0.5 mm for experiment. The content of in every flap is O.Smg. Put flap immerse into 20ml physiological saline, place in 37 癈 thermostat at illumination condition, to measure luminosity value by ultraviolet-visible spectrophotometer everyday last one month. Change 20ml physiological saline after every measure. Measured value is be analyzed by statistics.Result: the lower the ratio DL-PLA to CHI, the faster the Methylcobalamin release . Methylcobalamin release concentration of compound membrane(DL-PLA/ CHI =7/3) decrease observably every day and can not be detected at the 20 day. Methylcobalami...
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