Expression Of Multiple Factors In The Digestive Tract Of Portal Hypertensive Rats

The portal hypertension causes not only esophageal varices, but also gastric mucosal lesions, colonic mucosal lesions and enteric mucosal lesions, ect. Though great achievement in diagnosis and treatment of esophageal varices has been taken during last century, we even don't clearly understand the function of body factors in the local lesions of the digestive tract with portal hypertension. In this research, we first detected the expression of multiple factors in esophagus, stomach, small intestine and colon of portal hypertensive rats, in order to explore the local pathophysiological changes in the digestive tract.This research includes four parts.[ Part one ] Establishment of rat model of esophageal varices with portal hypertensionObjective To establish a rat model of esophageal varices with portal hypertension. Methods Fourty male SD rats were divided into two groups randomly, the experimental group in which a two-stage ligation of portal vein plus ligation of the left adrenal vein was performed, and the control (sham operation). Results Two weeks after complete portal ligation, the portal pressure and spleen weight in experimental group were significantly higher than the control. Compared with the control, the number of veins and the vessel area in esophageal submucosa increased significantly. Conclusions A rat model of esophageal varices with portal hypertension can be established with two-stage ligation of portal vein plus ligation of the left adrenal vein.[ Part two ] Dynamic expression of multiple factors in the development of esophageal varices in portal hypertensive ratsObjective To explore the dynamic expression of multiple factors in the development of esophageal varices in portal hypertensive rats. Methods The SD rats in experimental group, in which the two-stage ligation of portal vein plus ligation of the left adrenal vein was performed, were divided into three groups (S7, SI4, S21) at 7 days, 14 days and 21 days after complete portal ligation. The control was also separated intothree groups (D7, D14, D21 ) corresponed to the experimental. We detected the dynamic expression of iNOS, ecNOS, ET-K SP, CGRP> TNF-a > VEGF and PCNA in the esophagus of those six groups rats with immunohistochemical Strephavidin-Peroxidase technique. Results Compared with the controls, the expression of iNOS and ecNOS in three experimental groups were stronger, ET-1 and PCNA were stronger in S14 and S21,.while TNF-a and VEGF in S21. But SP and CGRP were found no different between experimental groups and the controls. Conclusions In the development of esophageal varices in portal hypertensive rats, increased NO may be early incident and the key to induce another factors in local, while ET-l and VEGF possibly play a role in the persistent phase. TNF-a may be play a role on the damaged mucosal state , SP and CGRP play no role as neural factor.[ Part three ] Dynamic expression of multiple factors in portal hypertensive gastric mucosa of ratsObjective To explore the dynamic expression of multiple factors in gastric mucosa of portal hypertensive rats. Methods The SD rats in experimental group 梪nderwent two-stage ligation of portal vein plus ligation of the left adrenal vein, were divided into three groups (S7, SI 4, S21) at 7 days, 14 days and 21 days after complete portal ligation. The control was also separated into three groups (D7, D14, D21 ) corresponed to the experimental. We detected the dynamic expression of iNOS> ecNOS, ET-K SP> CGRP> TNF-a, VEGF and PCNA in the gastric mucosa of those six groups rats with immunohistochemical Strephavidin-Peroxidase technique. Results Compared with the controls, the expression of iNOS, ecNOS, TNF-a and PCNA in three experimental groups were stronger, and ET-1 was stronger in SI4, while SP, CGRP and VEGF were stronger in S14 and S21. Conclusions In the portal hypertensive gastric lesion of rats, increased NOS and TNF-a may be early incident and play important role in the development , the increase of ET-1 may be transitory. SP and CGRP as neur...