The Effects Of SHENLUONING On Tubulointerstitial Lesions Of Experimental IgA Nephrosis Model Rat

Objective:IgA nephrosis (IgAN) is one of the most common pathologic types of primary glomerular diseases, there are about 50% patients with IgAN going to the end stage renal failure. Recently, with the further studies, people realized the proceeding deterioration of renal function has intimate correlation with tubulointerstitial lesions. The aim of the following research is to investigate the effects of Shenluoning (SHLN) formula on cytokines and extracellular matrix metabolism in tubulointerstitial tissue of IgAN model rats and discuss the action mechanism of the formula preventing and curing the pathologic changes in tubulointerstitial tissue.Method:Replicating IgAN rats model by taking orally and time-controlled vein injecting bovine serum albumin (BSA) and staphylococcus enterotoxin B (SEE), giving SHLN as treatment. At the same time Shenyankangfupian (SHYKFP) act as reference group, to observe blood urine nitrogen (BUN), serum creatinine (SCr), urine β-D-N-Acetyl glucosaminidase (NAG), 24 hours urine protein quantity, kidney morphology, ultrastructure and immune complex deposition. Using in situ hybridization(ISH), immunohistochemistry and computer image analysis to observe the effects of SI'LN on the expression of transforming growth factor β1(TGFβ1), collagen type III(Col-III), plasminogen activator inhibitor-1 (PAI-1), matrix metalloproteases-1 (MMP-1) and tissue inhibitors of matrixmetalloproteases-1 (TIMP-1) in tubulointerstitial of IgAN model rats.Results:(1) The contents of BUN, SCr, 24 hours urine protein quantity and urine NAG in SHLN group remarkablely decreased comparing with that in model group (P<0. 01 )0 (2) The result of Masson stain computer image analysis indicates the area ratio value of tubular cavity and tubule of model group is lower than that of normal group (P<0. 01) , SHLN group and SHYKFP group is better than model group (P<0. 01) , moreover, SHLN group is better than SHYKFP group (P<0. 05) .(3) In interstitial, the mRNA expression of TGFβ1, Col-III, PAI-1, TIMP-1 of model group are higher than that of normal group (P<0. 01) , SHLN group and SHYKFP group is better than model group (P<0. 01) and SHLN group is better than SHYKFP group (P<0. 05) .(4) Among the four groups, the expression of MMP-lmRNA of model group is little higher than normal group (P<0. 01) , SHLN group and SHYKFP group are obviously higher than model group (P<0. 01 or P<0. 05) , moreover, SHLN group is better than SHYKFP group (P<0. 05) .Conclusions:(1) SHLN is one of the effective formula for IgAN and tubulointerstitial lesions because the formula can decrease BUN, SCr, urine protein and urine NAG of IgAN model rats. (2) SHLN can release tubulointerstitial pathologic injury of IgAN model rats. (3) The above-mentioned actions may be relative with that SHLN can down-regulate the expression of TGF β1, Col-III, PAI-1, TIMP-1 and up-regulate that of MMP-1, those may be the mechanisms of SHLN preventing and curingtubulointerstitial lesions of IgAN.