The Clinical Study And The Experimental Study On The Mechanism Of Marrow-Supplementing And Blood-Engendering Granule To Related Adhesion Molecule In Chronic Aplastic Anemia Patients

For discussing the mechanism of marrow-supplementing and blood-engendering granule to chronic aplastic anemia (CAA) patients, this study employed single blind procedure to divide sixty CAA patients into marrow- supplementing and blood-engendering granule group and ZaiZhang ShengXue tablet group randomly and made observation and experiment on its clinical curative effect, live bone marrow examination constitution, bone marrow stromal cell (BMSC) adhesion molecule and adhesion function.This experiment employed iminumofluorescence method to examine the expression level of plalete endothelial cell adhesion molecule (PECAM/CD31), lymphocyte homing receptor(HCAM/CD44), intercellular adhesion molecule ( ICAM/CD54 ) , vascular cell adhesion molecule ( VCAM/ CD106) antigen in BMSC of CAA patients before and after treatment. This experiment used MMT method to test the adhesion function of BMSC in CAA patients to the normal and CAA patients bone hemopoietic cells.Result: 1. The effect of marrow-supplementing and blood-engendering granule to CAA was superior to that of ZaiZhang ShengXue tablet. In the marrow-supplementing -and blood-engendering granule group, its curative effect was superior in Yang deficiency type to that in Yin deficiency type, and the recovery of adhesion function of BMSC in Yang deficiency type was easier than that in Yin deficiency type. 2. Marrow-supplementing and blood-engendering granule can ameliorate pathologic status in many tissues in bone marrow, increase the area of hematopoietic tissue, redcuce adipose tissue, raise the amount of small vessels, and recover hematopoietic function in bone marrow. 3. The content of PECAM/CD31, HCAM/CD44,ICAM/CD54, VCAM/CD106 adhesion molecule in patients of the two groups were lower than that in the normal controlled group markedly before treatment. After treatment, the content of PECAM/CD31 , HCAM/CD44 , ICAM/CD54 , VCAM/CD106 antigen in two groups had all been advanced in different digree, and the advancement of PECAM/CD31, VCAM/CD106 antigen in group group. 4.The adhesion function of BMSC in CAA patients to bone hemopoietic cells descended markedly, after treatment the adhesion function in two groups was enhanced, and the enhancement in marrow-supplementing and blood-engendering granule group was superior to that in ZaiZhang ShengXue tablet group.Conclusion: The effect of marrow-supplementing and blood-engendering granule to CAA is superior to that of ZaiZhang ShengXue tablet. Our experiments further testified the theory 'Yin deficiency is easy to treat, but Yang deficiency is difficult to treat.' Marrow-supplementing and blood-engendering granule can ameliorate pathologic status in many tissues in bone marrow, and recover hematopoietic function in bone marrow.The expression level of adhesion molecule PECAM/CD31 , HCAM/CD44, ICAM/CD54 , VCAM/CD106 in CAA patients declined, and its adhesion function to bone hemopoietic cells also descended. Marrow-supplementing and blood-engendering granule can enhance expression level of adhesion molecule PECAM/CD31, HCAM /CD44, ICAM/CD54, VCAM/CD106 and the adhesion function of CAA BMSC to bone hemopoietic cells, therefore enhance the hemopoietic function of bone marrow.