| Pregnancy - induced hypertension ( PIH) is a kind of syndrome that often occurs in the pregnancy women after 20 weeks characterized by hypertentsion, proteinuria, edema and disfunction of many organs. Its incidence is about 7 -10% among the pregnant women. There are many doctrines about its pathogeny and transformation of its pathology. But there is not the verdict yet. So the approving methods relating to its prevention and therapy have not discovered up to the present. PIH is a sort of state that sympathetic nerve be activated. The excessive excitating state of the sympathetic nerve in PIH women has been confirmed by many experiments, and this state can depress immunol ability of the economy. In the course of physiology and pathology in PIH. The discharge of the sympathetic nerve system is reinforced, the tension of the blood vessels is strengthen, and goes to in gear. The current research consider: Pregnancy is a successful self - homogeneity transplanting phenomenon, its success depends on immunity balance among the mother and the fetus. Following the development of the procre-atioin immunology, the research of its immunological mechanism will be given more and more attention.At present, in clinical treatment of PIH, the obsterical department adopts the integerated methods that gives prioriyty to freeing from spasm by MgSO4 assists in making blood pressure fallen, calm, diuresis and ending pregnancy in timing, the effects in controlling treaing to clinical symptoms and complement are not quite pleasure. It has been reported that the continuous epidural blook is applied to treat PIH, and gets relatively well - pleasure effects, but short of detailed study and illustrating to mechanism, so as to influence its abroad applica-tion. In our department, Zhang Iihong had found that transection of the cervical sympathetic trunk (TCST) take on preventive and therapeutic effect on the rat with PIH, it shows there is of entire possible to search the therapy method. First time I apply the lumbar continuous epidural blook in rats with PIH, probe the methanism and the preventive and therapeutic effect on the rat with PIH, provide bascial evidence theory and experiment for clinical treatment og PIH, and makes it turn into a new technique that prevent and treat PIH.The study consists of three parts : 一. Preventive and therapeutic effects of the lumbar continuous epidural blook in rats with PIH. 二. the lumbar continuous epidural blook impact to Fas/FasL system and immunological function of CD4,CD8 lymphocyte cell. 三. the lumbar continuous epidural blook impacts to IL - 10 ,TNF - aof the rats with PIH.Materials and methods一 MaterialsAnimals :40 female Wistar rats weighting between 230 - 300g are provided by the animal lab of the second hospitol of China Medical University. They are breed in barrier system room tempreture 18 -28T1 and relative humidity 40 -70%.Mainapparatus: The artery munnometer of the rat tail ( RBP - 1). Electrial scale AA - 250. DuBang low temperature and highspeed centrifuger.HEIDOLPH DIAX900 neat slurry engine. S500 - P ultraviolet the visible continuous spectrometer. UP200H ultrasonic muller. PCR amplification device. OLMPUS B x 60 photomicrography Metamorph microscope image analyzer. SUNRISE enzyme marking instrument.Main Reagent: L - nitroarginomethyl ester ( L - NAME). CD4, CD8 Im-munohistochemical detection kit (Boster). CD4, CD8 reverse trans - cription kits (TaKaRa). The rat IL - 10 ration EIA kit. The rat TNF - aration EIA kit. 二 Experimental Methods1 Animal model: Consulting Olson's methods, make some progress based on practice, replicate PIH rat model. Pregnant rats are put catheter into epiduralcavity and preserved for a long time. Anesthesia: Inject 10% hydration chlori-dine aldehyde (4ml/kg) into the abdominal space of the rats, the rats commonly elope off and fall down in 4 - 6 minutes. The course of operation: The animal is taken bow and lying position,a cylinder that diameter equal to 2. 5cm is layed under the waist, limb and head ar...
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