| Accordingt to the pathogenic pecularity of retarded circulation of blood duing to qi deficiency and residual poison which is not being removed, Ping-Yi (PY)Mixture based on the therapeutic principle of invigorating qi to promoting blood circulation and eliminating poison to dissipate lumps was studied out for postoprative tumor patients, combined clinic with chemotherapy to observe the influence on relapse and metastasis rate within 1 or 2 years for period II ~IIIpostoprative tumor patients, and compared with control group which was applied chemotherapy methods only. The results showed that the metastasis and relapse rate within 1 or 2 years of treatment group was respectively 25.74% and 31.43%,which was statistically significiently different from that of control group which was respectively 32.00% and 52. 00%(P<0. 05), the survivle rate of treatment group was 91.43% and 78.57% respectively, which was statistically significiently different from that of control group which was 77. 57% and 58. 00% respectively, so that there was significent differece between above two groups(P<0.05).Also the clinical research showed that PY could improve the quality of livings , adjust disequilibrium of T-cell subgroup, transform the hemorheology, and alleviate the ill-effect of chemotherapy, there was significent differece between above two groups (P<0. 05~0.01).Animal experiment proved that PY Mixture had evident inhibition for Lewis lung cancer, H22 hepatocarcinoma trasplanted tumor, hematogenous and lymphatic metastasis of experimental animal, as well as the growth of pulmonic transplanted tumor, and was different from that of control group on metastasis rate and level of H22 hepatocarcinoma lymphatic metastasis, pulmonic and lymphatic metastasis of postoprative Lewis lung cancer (P<0. 05~0.01), and could reduce the MVD in the tumor constitution and its stroma ,inhibited the expression of VEGF in the tumor. The effect of mass-dosage group was superior to that of adequacy-dosage group, and adequacy-dosage combining withchemotherapy group had signif icent action to increase above effect, to protect the immune orgnism of the tumor mice , and to reduce the impairment of the immune orgnism caused by chemotherapy.In vitro reseach utilizing the method of serum pharmacolog, selected different concentration of serum which included PY, incubating with PG-cell.The results was observed asynchronously, and compared with blank control group. It showed that:the serum which include PY had evidently inhibited effect on the growth of in vitro-colture PG-cell, which was enhanced with the increation of drug-concentration in serum and the elongation of action time. The inhibition of 24nd hour, 48nd hour and 72nd hour of the effect of 20% drug-serum is 37. 38%, 38.45%, 42.61% respectively, and together with DDP .could signif icently increase the ADCC of DDP for PG-cell, Compared with DDP group, every PY-DDP group had evident difference from it(P<0.05~0. 01). In vitro reseach showed that serum which include PY could evidently inhibited the expression of angiogenesis factor which was promoted by PG-cellsVEGF, bFGF and MMP-9, regulating the expression of mutation P53gene down, blocking the carcinomic cells in periodG0-Giand inducing apoptosis of carcinomic cells, and the effection was enhanced with the increation of drug-concentration in serum and the elongation of action time.Clinnical and experimental research showed that PY had the effection of anti-tumor and anti-metastasis, which could interupt multilinks in the process of angiogensis and control the malignant progress of metastasis by several ways include: (1) reducing the MVD in the tumor constitution and its stroma; (2) inhibiting the expression of angiogenesis factor VEGF, bFGF, MMP-9; (3) regulating the expression of mutation P53 gene down; (4) indue ing apoptosis of carcinomic cells; (5)protecting the immune apparatus and enhancing abilities of immunocytes; (6)reducing blood viscosity and improving the powful-viscidity status and so on. The research combinded with serum pharmacolog s... |
PDF Full Text Request