The Study Of The Mechanism Of That The Reinforcing Kidney And Exhausting Toxin Mixture Remedy CRF Malnutrition

Objective:To investigate the mechanism of malnutrition in chronic renal failure (CRF) and observe the adjusting effect by the method of promoting kidney and spleen , regulating pungative and exhausting toxin. Methods: Combining the Traditional Chinese Medicine theory to the modern molecule-biology , putting forward the hypothesis that CRF malnutrition are resulted from spleen and kidney asthenia,Blocking of tri-energizer,Tetention of damp and stasis of blood; turbid pathogens in the body.Take invigorating spleen and tonifying kidney, mediate econnomy's function and exhauste toxin as treatment method, composing the formula rationally, choosing the medicine carefully, to make the Reinforcing Kidney and Exhausting Toxin Mixrure(REM). Method:The 60 wistar rats was divided into 3 groups randomly: the normal contral group(group I ),CRF group(group II ),and CRF rat perfusing with REM group(group III).Observing the weight change of the rat and their biochemical results; Taking their fat kidney brain tissue for detecting the gene and protein expression of the leptin leptin receptor(Ob-Ra,Ob-Rb),and neuropeptide Y (NPY) by the means of RT-PCR Southern bloting In situ hybridization and immunohistochemistry.Result: Comparing with CRF group, the rats treated by REM have higher weight and ALB level and their anaemia was more gently also .The leptin gene expression decreases in CRF group ,while the protein exprese intensely;In brain tissue ,the expression of Ob-Ra Ob-Rb enhanced and NPY was inhabited; In kidney tissue,the Ob-Ra expressed weakly. After perfusing with the REM, the Ob-Ra Ob-Rb expression level was inhibited,which make NPY was reinforced in brain; renal ob-ra expressed more strongly than CRF group.Conclusion: It is the main mechanism of CRF malnutrition ,that is Spleen and kidneyasthenia short of regulation of ShaoYang, hygric retention and blood stasis and storage of toxin in the body. Promoting kidney and spleen , regulating pungative and exhausting toxin was a important treatment method, There are metabolic disorder in leptin-neuroendocrine axis in CRF rats and it is related with nutrient state . Maybe the REM could do a little better with the malnutrition of CRF rats by regulating the activity of Ob-ra, Ob-rb in brain.