Experimental Research On Delaying Aging By Four Noble Drugs

The year of 1999 is the International Old Age Year designated by the UN Conference. This indicates that the problem of old age has aroused the universal concern and emphasis of the international society. To enforce the research on the principle of aging and delaying aging is an important research subject in the medical field in the new century. Traditional Chinese medicine has a bright future in delaying aging.Traditional Chinese Medicine thinks that people's aging is closely related to Zang, Fu, channels, collateral. Qi, blood. Yin. Yang and the vigor. Between channels and collateral, kidney and spleen are most related to each other. Spleen is the source of Qi and blood. It is responsible for carrying and sending up and is closely related to the body's growth and aging. The deficiency of the spleen is the cause of aging and is also the pathological process in aging. As a resuct. invigorating spleen can delay aging. Decoction of Four Noble Drugs is the representative recipe of strengthening the spleen and replenishing Qi. Its elements are all superior ones included in Shen Nong's Herbal Classic and have the function of expelling the diseases and lengthening one's life. Modern pathological research also proves that Decoction of Four Noble Drugs can promote the activity of antioxidase of the body and strengthen the immunity of the body. Based on previous research as well as the theory of free radical and the theory of telemeter, this article does further research on the Decoction of Four Noble Drugs in its delaying aging mechanism so as to provide proof for the theory of deficiency of spleen and aging and therefore lay a good basis for developing the Decoction of Four Noble Drugs as a drug of delaying aging.The main contents of the research.I. The influence of the Decoction of Four Noble Drugs on the memorizing ability of mice in the D-gal aging model.Use the mice jumping-platform method and observ and record the time that the mice stay in the safe area, in the wrong area, the latent time and the number of mistakes.II. The influence of Decoction of Four Noble Drugs on the activity of superoxide dixmutase in the tissues of the mice's heart, brain, and liver.Use the MTT method and test the SOD activity of the tissues of the mice's heart, brain and liver and compare statistically the SOD activity changes of the tissues of the heart, brain and liver of different groups of mice.III. The influence of the Decoction of Four Noble Drugs on the activity of gluts throne peroxides in the mice's liver tissues in the D-gal aging model.Use the DTNB method and test the GSH-Px activity in the liver tissues of various groups of mice. Then compare statistically the GSH-Px activity changes in the liver tissues of various groups of mice.IV. The influence of the Decoction of Four Noble Drugs on lipid peroxides decomposed product, i. e MDA contentUse the TBA colorimeter and test the MDA content of the tissues of heart, brain and liver of various groups of mice.V. The influence of the Decoction of Four Noble Drugs on telemeter enzyme activity of the tissues of the heart, brain, liver and thymus gland of the mice in the D-gal aging model.Use the PCR-ELISA method and test the telemeter enzyme activity of the telemeter enzyme activity of the tissues of the heart, brain, liver and thymus gland of the mice and compare statistically the changes of the telemeter enzyme activity of the tissues of the heart, brain, liver and thymus gland of the various groups of mice.VI. The influence of the Decoction of Four Noble Drugs on the mice's brain tissue morphology in the D-gal aging model.The research result shows that:I. Different dosage groups of the Decoction of Four Noble Drugs can improve the mice's learning and memorizing ability, especially improve the mice's learning and memorizing ability in the high-dosage group in the D-gal aging model.II. Different dosage groups of the Decoction of Four Noble Drugs can improve the mice's heart, brain and liver tissue SOD activity, especially the high-dosage whic...